Identification of suppressive components in "Haemophilus somnus" fractions which inhibit bovine polymorphonuclear leukocyte function

dc.contributor.author Chiang, Yu-Wei
dc.contributor.author Kaeberle, Merlin
dc.contributor.author Roth, James
dc.contributor.author Roth, James
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-04-12T18:47:07.000
dc.date.accessioned 2020-07-07T05:14:53Z
dc.date.available 2020-07-07T05:14:53Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 1986
dc.date.issued 1986-06-01
dc.description.abstract <p>"Haemophilus somnus" fractions which inhibited iodination of protein by bovine polymorphonuclear leukocytes were isolated by heat extracting a washed bacterial suspension at 60 degrees C or incubating the bacterial suspension at 37 degrees C and were partially purified by ultrafiltration. The components in each fraction were separated by reverse-phase high-performance liquid chromatography and identified as ribonucleotides, a ribonucleoside, and purine and pyrimidine bases. Most of the compounds were found to be inhibitory to iodination in a dose-dependent manner. When the effect of each component on iodination at the concentrations present in "H. somnus" fractions was determined, it was found that guanine and GMP were the components responsible for most of the suppression in the fraction isolated by heat extraction, whereas guanine and adenine were the major inhibitory components in the fraction isolated by incubation at 37 degrees C.</p>
dc.description.comments <p>This article is published as Chiang, Y. W., M. L. Kaeberle, and J. A. Roth. "Identification of suppressive components in" Haemophilus somnus" fractions which inhibit bovine polymorphonuclear leukocyte function." <em>Infection and immunity</em> 52, no. 3 (1986): 792-797. Posted with permission.</p>
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dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/189/
dc.identifier.articleid 1193
dc.identifier.contextkey 11951851
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/189
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92297
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/189/1986_Roth_IdentificationSuppressive.pdf|||Fri Jan 14 21:47:37 UTC 2022
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.title Identification of suppressive components in "Haemophilus somnus" fractions which inhibit bovine polymorphonuclear leukocyte function
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 909dd0b2-ec0a-41e2-b4e0-9e5ff76b7622
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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