Small non-coding RNA CjNC110 influences motility, autoagglutination, AI-2 localization, and chicken colonization in Campylobacter jejuni

dc.contributor.author Plummer, Paul
dc.contributor.author Kreuder, Amanda
dc.contributor.author Ruddell, Brandon
dc.contributor.author Mou, Kathy
dc.contributor.author Hassall, Alan
dc.contributor.author Zhang, Qijing
dc.contributor.author Zhang, Qijing
dc.contributor.author Plummer, Paul
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2020-01-10T17:06:23.000
dc.date.accessioned 2020-07-07T05:15:02Z
dc.date.available 2020-07-07T05:15:02Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2020
dc.date.issued 2020-01-03
dc.description.abstract <p>Small non-coding RNAs are involved in many important physiological functions in pathogenic microorganisms. Previous studies have identified the presence of non-coding RNAs in the major zoonotic pathogen <em>Campylobacter jejuni</em>, however, few have been functionally characterized to date. CjNC110 is a conserved ncRNA in <em>C. jejuni,</em> located downstream of the <em>luxS</em> gene which is responsible for the production of the quorum-sensing molecule autoinducer-2 (AI-2). In this study, we utilized strand specific high-throughput RNAseq to identify potential targets or interactive partners of CjNC110 in a sheep abortion clone of <em>C. jejuni</em>. This data was utilized to focus further phenotypic evaluation of the role of CjNC110 in growth, motility, autoagglutination, quorum sensing, and chicken colonization in <em>C. jejuni</em>. Inactivation of the CjNC110 ncRNA led to a statistically significant decrease in autoagglutination ability as well as increased motility when compared to wild-type. Extracellular AI-2 detection was decreased in ΔCjNC110, however, intracellular AI-2 accumulation was significantly increased with a concurrent increase in LuxS expression, suggesting a key role of CjNC110 in modulating the transport of AI-2. Notably, ΔCjNC110 also showed a significant defect in the ability to colonize chickens. Complementation of CjNC110 restored all phenotypic changes back to wild-type levels. The collective results of the phenotypic and transcriptomic changes observed in our data provide valuable insights into the pathobiology of <em>C. jejuni</em> sheep abortion clone and strongly suggest that CjNC110 plays an important role in regulation of energy taxis, flagellar glycosylation, cellular communication via quorum sensing, and chicken colonization in this important zoonotic pathogen.</p>
dc.description.comments <p>This is a pre-print of the article Kreuder, Amanda J., Brandon Ruddell, Kathy Mou, Alan Hassall, Qijing Zhang, and Paul J. Plummer. "Small non-coding RNA CjNC110 influences motility, autoagglutination, AI-2 localization, and chicken colonization in Campylobacter jejuni." <em>bioRxiv</em> (2020). DOI: <a href="http://dx.doi.org/10.1101/2020.01.02.893479" target="_blank">10.1101/2020.01.02.893479</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/210/
dc.identifier.articleid 1209
dc.identifier.contextkey 16171047
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/210
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92322
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/210/2020_ZhangQijing_SmallNoncoding.pdf|||Fri Jan 14 22:33:58 UTC 2022
dc.source.uri 10.1101/2020.01.02.893479
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.title Small non-coding RNA CjNC110 influences motility, autoagglutination, AI-2 localization, and chicken colonization in Campylobacter jejuni
dc.type article
dc.type.genre article
dspace.entity.type Publication
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