Sensitive PCR Method for Detection of Escherichia coli 0157:H7 and Other Shiga toxin-producing Bacteria in Ground Meat
dc.contributor.author | Matise, I. | |
dc.contributor.author | Shelton, M. | |
dc.contributor.author | Phillips, G. | |
dc.contributor.author | Will, L. | |
dc.date | 2018-02-13T00:29:22.000 | |
dc.date.accessioned | 2020-07-02T07:00:12Z | |
dc.date.available | 2020-07-02T07:00:12Z | |
dc.date.copyright | Thu Jan 01 00:00:00 UTC 1998 | |
dc.date.issued | 1998 | |
dc.description.abstract | <p>Sensitivity of a polymerase chain reaction (PCR) procedure was evaluated for Escherichia coli O157:H7 and Shiga toxin gene (stx) detection in ground beef and ground pork at contamination levels of 0.14, 1.4, and 14 colony forming units per gram (CFU/g) of meat. The PCR procedure, developed during our previous research, amplifies three target genes simultaneously: uidA that is specific for E. coli O157:H7, and stx1 and stx2, the genes for Shiga toxins 1 and 2, respectively. Detection of the uidA gene by the PCR was 91% sensitive in beef and 55% sensitive in pork, and 90% and 71% sensitive for stx gene detection in beef and pork, respectively. In comparison, detection of E. coli O157:H7 by culture, done simultaneously with PCR, was 53% sensitive in beef and 11% sensitive in pork. Results indicate that this PCR procedure is a rapid and sensitive method for STEC and E. coli O157:H7 detection in meat at contamination levels less than 1 CFU/g.</p> | |
dc.format.mimetype | application/pdf | |
dc.identifier | archive/lib.dr.iastate.edu/swinereports_1997/33/ | |
dc.identifier.articleid | 1032 | |
dc.identifier.contextkey | 3233645 | |
dc.identifier.s3bucket | isulib-bepress-aws-west | |
dc.identifier.submissionpath | swinereports_1997/33 | |
dc.identifier.uri | https://dr.lib.iastate.edu/handle/20.500.12876/91076 | |
dc.language.iso | en | |
dc.relation.ispartofseries | Animal Science Research Reports | |
dc.relation.ispartofseries | ASL R1509 | |
dc.source.bitstream | archive/lib.dr.iastate.edu/swinereports_1997/33/asl_1509.pdf|||Fri Jan 14 23:37:15 UTC 2022 | |
dc.subject.disciplines | Agriculture | |
dc.subject.disciplines | Animal Sciences | |
dc.subject.keywords | ASL R1509 | |
dc.title | Sensitive PCR Method for Detection of Escherichia coli 0157:H7 and Other Shiga toxin-producing Bacteria in Ground Meat | |
dc.type | article | |
dc.type.genre | health | |
dspace.entity.type | Publication | |
relation.isSeriesOfPublication | 7f3839b7-b833-4418-a6fa-adda2b23950a |
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