Sensitive PCR Method for Detection of Escherichia coli 0157:H7 and Other Shiga toxin-producing Bacteria in Ground Meat

dc.contributor.author Matise, I.
dc.contributor.author Shelton, M.
dc.contributor.author Phillips, G.
dc.contributor.author Will, L.
dc.date 2018-02-13T00:29:22.000
dc.date.accessioned 2020-07-02T07:00:12Z
dc.date.available 2020-07-02T07:00:12Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 1998
dc.date.issued 1998
dc.description.abstract <p>Sensitivity of a polymerase chain reaction (PCR) procedure was evaluated for Escherichia coli O157:H7 and Shiga toxin gene (stx) detection in ground beef and ground pork at contamination levels of 0.14, 1.4, and 14 colony forming units per gram (CFU/g) of meat. The PCR procedure, developed during our previous research, amplifies three target genes simultaneously: uidA that is specific for E. coli O157:H7, and stx1 and stx2, the genes for Shiga toxins 1 and 2, respectively. Detection of the uidA gene by the PCR was 91% sensitive in beef and 55% sensitive in pork, and 90% and 71% sensitive for stx gene detection in beef and pork, respectively. In comparison, detection of E. coli O157:H7 by culture, done simultaneously with PCR, was 53% sensitive in beef and 11% sensitive in pork. Results indicate that this PCR procedure is a rapid and sensitive method for STEC and E. coli O157:H7 detection in meat at contamination levels less than 1 CFU/g.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/swinereports_1997/33/
dc.identifier.articleid 1032
dc.identifier.contextkey 3233645
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath swinereports_1997/33
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91076
dc.language.iso en
dc.relation.ispartofseries Animal Science Research Reports
dc.relation.ispartofseries ASL R1509
dc.source.bitstream archive/lib.dr.iastate.edu/swinereports_1997/33/asl_1509.pdf|||Fri Jan 14 23:37:15 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.keywords ASL R1509
dc.title Sensitive PCR Method for Detection of Escherichia coli 0157:H7 and Other Shiga toxin-producing Bacteria in Ground Meat
dc.type article
dc.type.genre health
dspace.entity.type Publication
relation.isSeriesOfPublication 7f3839b7-b833-4418-a6fa-adda2b23950a
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