Single cell transcriptome profiling of developing chick retinal cells Laboissonniere, Lauren Trimarchi, Jeffrey Martin, Gregory Goetz, Jillian Bi, Ran Pope, Brock Weinand, Kallie Ellson, Laura Fru, Diane Lee, Miranda Wester, Andrea Liu, Peng Trimarchi, Jeffrey
dc.contributor.department Statistics
dc.contributor.department Genetics, Development and Cell Biology 2018-02-17T13:00:08.000 2020-06-30T04:01:41Z 2020-06-30T04:01:41Z Sun Jan 01 00:00:00 UTC 2017 2018-08-15 2017-08-15
dc.description.abstract <p>The vertebrate retina is a specialized photosensitive tissue comprised of six neuronal and one glial cell types, each of which develops in prescribed proportions at overlapping timepoints from a common progenitor pool. While each of these cells has a specific function contributing to proper vision in the mature animal, their differential representation in the retina as well as the presence of distinctive cellular subtypes makes identifying the transcriptomic signatures that lead to each retinal cell’s fate determination and development challenging. We have analyzed transcriptomes from individual cells isolated from the chick retina throughout retinogenesis. While we focused our efforts on the retinal ganglion cells, our transcriptomes of developing chick cells also contained representation from multiple retinal cell types, including photoreceptors and interneurons at different stages of development. Most interesting was the identification of transcriptomes from individual mixed lineage progenitor cells in the chick as these cells offer a window into the cell fate decision-making process. Taken together, these datasets will enable us to uncover the most critical genes acting in the steps of cell fate determination and early differentiation of various retinal cell types.</p>
dc.description.comments <p>This is the peer reviewed version of the following article: Laboissonniere, Lauren A., Gregory M. Martin, Jillian J. Goetz, Ran Bi, Brock Pope, Kallie Weinand, Laura Ellson et al. "Single cell transcriptome profiling of developing chick retinal cells." <em>Journal of Comparative Neurology</em> (2017) which has been published in final form at doi: <a href="" target="_blank">10.1002/cne.24241</a>. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.</p>
dc.format.mimetype application/pdf
dc.identifier archive/
dc.identifier.articleid 1187
dc.identifier.contextkey 11570324
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/183
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 21:40:00 UTC 2022
dc.source.uri 10.1002/cne.24241
dc.subject.disciplines Cell Biology
dc.subject.disciplines Developmental Biology
dc.subject.disciplines Genetics
dc.subject.disciplines Statistical Models
dc.subject.keywords retina
dc.subject.keywords single cell transcriptomics
dc.subject.keywords ganglion cells
dc.subject.keywords development
dc.title Single cell transcriptome profiling of developing chick retinal cells
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 6e9f21d0-82ad-4661-a8f7-7634953c036b
relation.isOrgUnitOfPublication 264904d9-9e66-4169-8e11-034e537ddbca
relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
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