The maize (Zea mays L.) roothairless3 gene encodes a putative GPI-anchored, monocot-specific, COBRA-like protein that significantly affects grain yield

dc.contributor.author Hochholdinger, Frank
dc.contributor.author Wen, Tsui-Jung
dc.contributor.author Lamkey, Kendall
dc.contributor.author Chimot-Marolle, Patricia
dc.contributor.author Da Costa e Silva, Oswaldo
dc.contributor.author Bruce, Wesley
dc.contributor.author Lamkey, Kendall
dc.contributor.author Wienand, Udo
dc.contributor.author Schnable, Patrick
dc.contributor.department Agronomy
dc.date 2018-02-16T07:43:52.000
dc.date.accessioned 2020-06-29T23:05:10Z
dc.date.available 2020-06-29T23:05:10Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2008
dc.date.issued 2008-06-01
dc.description.abstract <p>The <em>rth3</em> (<em>roothairless 3</em>) mutant is specifically affected in root hair elongation. We report here the cloning of the <em>rth3</em> gene via a PCR-based strategy (amplification of insertion mutagenized sites) and demonstrate that it encodes a COBRA-like protein that displays all the structural features of a glycosylphosphatidylinositol anchor. Genes of the <em>COBRA</em> family are involved in various types of cell expansion and cell wall biosynthesis. The <em>rth3</em> gene belongs to a monocot-specific clade of the <em>COBRA</em> gene family comprising two maize and two rice genes. While the rice (<em>Oryza sativa</em>) gene <em>OsBC1L1</em> appears to be orthologous to <em>rth3</em> based on sequence similarity (86% identity at the protein level) and maize/rice synteny, the maize (<em>Zea mays</em> L.) <em>rth3-like</em> gene does not appear to be a functional homolog of <em>rth3</em>based on their distinct expression profiles. Massively parallel signature sequencing analysis detected <em>rth3</em> expression in all analyzed tissues, but at relatively low levels, with the most abundant expression in primary roots where the root hair phenotype is manifested.<em>In situ</em> hybridization experiments confine <em>rth3</em> expression to root hair-forming epidermal cells and lateral root primordia. Remarkably, in replicated field trials involving near-isogenic lines, the <em>rth3</em> mutant conferred significant losses in grain yield.</p>
dc.description.comments <p>This article is from <em>The Plant Journal</em> 54 (2008): 888–898, doi:<a href="http://dx.doi.org/10.1111/j.1365-313X.2008.03459.x" target="_blank">10.1111/j.1365-313X.2008.03459.x</a>. Posted with permission.</p>
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dc.identifier archive/lib.dr.iastate.edu/agron_pubs/43/
dc.identifier.articleid 1041
dc.identifier.contextkey 7031688
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath agron_pubs/43
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/4787
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/agron_pubs/43/2008_Hochholdinger_MaizeZea.pdf|||Sat Jan 15 00:14:49 UTC 2022
dc.source.uri 10.1111/j.1365-313X.2008.03459.x
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Genetics
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.keywords rth3
dc.subject.keywords COBRA-like
dc.subject.keywords root hairs
dc.subject.keywords maize
dc.subject.keywords mutant
dc.title The maize (Zea mays L.) roothairless3 gene encodes a putative GPI-anchored, monocot-specific, COBRA-like protein that significantly affects grain yield
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication fadecd27-89f7-4164-8981-ce39d1a066e1
relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a
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