The maize (Zea mays L.) roothairless3 gene encodes a putative GPI-anchored, monocot-specific, COBRA-like protein that significantly affects grain yield
dc.contributor.author | Hochholdinger, Frank | |
dc.contributor.author | Wen, Tsui-Jung | |
dc.contributor.author | Lamkey, Kendall | |
dc.contributor.author | Chimot-Marolle, Patricia | |
dc.contributor.author | Da Costa e Silva, Oswaldo | |
dc.contributor.author | Bruce, Wesley | |
dc.contributor.author | Lamkey, Kendall | |
dc.contributor.author | Wienand, Udo | |
dc.contributor.author | Schnable, Patrick | |
dc.contributor.department | Agronomy | |
dc.date | 2018-02-16T07:43:52.000 | |
dc.date.accessioned | 2020-06-29T23:05:10Z | |
dc.date.available | 2020-06-29T23:05:10Z | |
dc.date.copyright | Tue Jan 01 00:00:00 UTC 2008 | |
dc.date.issued | 2008-06-01 | |
dc.description.abstract | <p>The <em>rth3</em> (<em>roothairless 3</em>) mutant is specifically affected in root hair elongation. We report here the cloning of the <em>rth3</em> gene via a PCR-based strategy (amplification of insertion mutagenized sites) and demonstrate that it encodes a COBRA-like protein that displays all the structural features of a glycosylphosphatidylinositol anchor. Genes of the <em>COBRA</em> family are involved in various types of cell expansion and cell wall biosynthesis. The <em>rth3</em> gene belongs to a monocot-specific clade of the <em>COBRA</em> gene family comprising two maize and two rice genes. While the rice (<em>Oryza sativa</em>) gene <em>OsBC1L1</em> appears to be orthologous to <em>rth3</em> based on sequence similarity (86% identity at the protein level) and maize/rice synteny, the maize (<em>Zea mays</em> L.) <em>rth3-like</em> gene does not appear to be a functional homolog of <em>rth3</em>based on their distinct expression profiles. Massively parallel signature sequencing analysis detected <em>rth3</em> expression in all analyzed tissues, but at relatively low levels, with the most abundant expression in primary roots where the root hair phenotype is manifested.<em>In situ</em> hybridization experiments confine <em>rth3</em> expression to root hair-forming epidermal cells and lateral root primordia. Remarkably, in replicated field trials involving near-isogenic lines, the <em>rth3</em> mutant conferred significant losses in grain yield.</p> | |
dc.description.comments | <p>This article is from <em>The Plant Journal</em> 54 (2008): 888–898, doi:<a href="http://dx.doi.org/10.1111/j.1365-313X.2008.03459.x" target="_blank">10.1111/j.1365-313X.2008.03459.x</a>. Posted with permission.</p> | |
dc.format.mimetype | application/pdf | |
dc.identifier | archive/lib.dr.iastate.edu/agron_pubs/43/ | |
dc.identifier.articleid | 1041 | |
dc.identifier.contextkey | 7031688 | |
dc.identifier.s3bucket | isulib-bepress-aws-west | |
dc.identifier.submissionpath | agron_pubs/43 | |
dc.identifier.uri | https://dr.lib.iastate.edu/handle/20.500.12876/4787 | |
dc.language.iso | en | |
dc.source.bitstream | archive/lib.dr.iastate.edu/agron_pubs/43/2008_Hochholdinger_MaizeZea.pdf|||Sat Jan 15 00:14:49 UTC 2022 | |
dc.source.uri | 10.1111/j.1365-313X.2008.03459.x | |
dc.subject.disciplines | Agricultural Science | |
dc.subject.disciplines | Agriculture | |
dc.subject.disciplines | Agronomy and Crop Sciences | |
dc.subject.disciplines | Genetics | |
dc.subject.disciplines | Plant Breeding and Genetics | |
dc.subject.keywords | rth3 | |
dc.subject.keywords | COBRA-like | |
dc.subject.keywords | root hairs | |
dc.subject.keywords | maize | |
dc.subject.keywords | mutant | |
dc.title | The maize (Zea mays L.) roothairless3 gene encodes a putative GPI-anchored, monocot-specific, COBRA-like protein that significantly affects grain yield | |
dc.type | article | |
dc.type.genre | article | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | fadecd27-89f7-4164-8981-ce39d1a066e1 | |
relation.isOrgUnitOfPublication | fdd5c06c-bdbe-469c-a38e-51e664fece7a |
File
Original bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- 2008_Hochholdinger_MaizeZea.pdf
- Size:
- 1.65 MB
- Format:
- Adobe Portable Document Format
- Description: