Correlating blood immune parameters and a CCT7 genetic variant with the shedding of Salmonella enterica serovar Typhimurium in swine

dc.contributor.author Uthe, J. J.
dc.contributor.author Nettleton, Dan
dc.contributor.author Wang, Y.
dc.contributor.author Qu, L.
dc.contributor.author Nettleton, Dan
dc.contributor.author Tuggle, C. K.
dc.contributor.author Bearson, S. M. D.
dc.contributor.department Animal Science
dc.contributor.department Statistics
dc.date 2019-08-24T14:19:54.000
dc.date.accessioned 2020-07-02T06:57:10Z
dc.date.available 2020-07-02T06:57:10Z
dc.date.issued 2009-03-30
dc.description.abstract <p>The porcine response to <em>Salmonella</em> infection is critical for control of <em>Salmonella</em> fecal shedding and the establishment of <em>Salmonella</em> carrier status. In this study, 40 crossbred pigs were intranasally inoculated with <em>Salmonella enterica</em> serovar Typhimurium (<em>Salmonella</em> Typhimurium) and monitored for <em>Salmonella</em> fecal shedding and blood immune parameters at 2, 7, 14 and 20 days post-inoculation (dpi). Using a multivariate permutation test, a positive correlation was observed between <em>Salmonella</em> Typhimurium shedding levels at 2 and 7 dpi and serum interferon-gamma (IFNγ) levels at 2 dpi (<em>p < </em>0.05), with <em>Salmonella</em> being shed in greater numbers from animals with higher IFNγ levels. A positive correlation was also observed between IFNγ levels and the number of banded neutrophils (2 dpi), circulating neutrophils (7 and 14 dpi), monocytes (7 dpi), and white blood cells (WBCs) (7, 14 and 20 dpi). We have further performed association studies on these immune response parameters as well as shedding status of the <em>Salmonella</em>-infected pigs with a single nucleotide polymorphism (SNP) in the porcine gene CCT7, previously shown by our group to be transcriptionally up-regulated in swine experimentally inoculated with <em>Salmonella</em> Typhimurium. Our analyses with the 40 pigs suggest a positive association (<em>p = </em>0.0012) of SNP genotype A/G at position AK240296.c1153G > A of the CCT7 gene with <em>Salmonella</em> shedding at 7 dpi compared to the G/G homozygote genotype. Linking specific genes and genetic polymorphisms with the porcine immune response to <em>Salmonella</em> infection and shedding may identify potential markers for carrier pigs as well as targets for disease diagnosis, intervention and prevention.</p>
dc.description.comments <p>This article is published as Uthe, J. J., Y. Wang, L. Qu, D. Nettleton, C. K. Tuggle, and S. M. D. Bearson. "Correlating blood immune parameters and a CCT7 genetic variant with the shedding of Salmonella enterica serovar Typhimurium in swine." <em>Veterinary microbiology</em> 135, no. 3-4 (2009): 384-388. doi: <a href="https://doi.org/10.1016/j.vetmic.2008.09.074" target="_blank" title="Persistent link using digital object identifier">10.1016/j.vetmic.2008.09.074</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/stat_las_pubs/205/
dc.identifier.articleid 1211
dc.identifier.contextkey 14851631
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath stat_las_pubs/205
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/90516
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/stat_las_pubs/205/2009_Nettleton_CorrelatingBlood.pdf|||Fri Jan 14 22:25:34 UTC 2022
dc.source.uri 10.1016/j.vetmic.2008.09.074
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Biostatistics
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.keywords Salmonella
dc.subject.keywords SwineInterferon-gamma (IFN-γ)
dc.subject.keywords Single nucleotide polymorphism (SNP)
dc.subject.keywords CCT7
dc.title Correlating blood immune parameters and a CCT7 genetic variant with the shedding of Salmonella enterica serovar Typhimurium in swine
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
relation.isOrgUnitOfPublication 264904d9-9e66-4169-8e11-034e537ddbca
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