The Turnip mosaic virus and its effects on Arabidopsis thaliana gene expression
dc.contributor.advisor | Steven A. Whitham | |
dc.contributor.author | Campbell, Brian | |
dc.contributor.department | Plant Pathology and Microbiology | |
dc.date | 2018-07-21T06:05:40.000 | |
dc.date.accessioned | 2020-06-30T02:46:59Z | |
dc.date.available | 2020-06-30T02:46:59Z | |
dc.date.copyright | Tue Jan 01 00:00:00 UTC 2013 | |
dc.date.embargo | 2015-07-30 | |
dc.date.issued | 2013-01-01 | |
dc.description.abstract | <p>Utilizing natural and engineered viruses is an accepted approach to studying plant-virus interactions as it relates to symptomology. The majority of the research topics were generated by deciphering where short-comings in the literature existed. Specifically, how Turnip mosaic virus (TuMV) helper component protease (HC-Pro) small RNA (sRNA) binding affinity affects expression of genes correlated with disease phenotypes and studying debilitated viruses in a variety of RNA silencing deficient Arabidopsis thaliana plants. Taken as a whole, the research presented addressed the susceptibility of A. thaliana to TuMV.</p> <p>The first study was conducted to monitor genes implicated in symptomology in various RNA silencing pathway mutant backgrounds. I hypothesized that an in vitro approach, in conjunction with an in silico study would reveal the mechanism TuMV utilizes to regulate sRNA expression, post-infection. The second study focused on severe, moderate, and weak TuMV strains, versus A. thaliana response to pathogen challenge. I hypothesized that TuMV HC-Pro FRNK box mutants that differed in their ability to infect plants affected the function of host sRNA in graduated steps. I also postulated that these mutants might allow me to uncouple developmental abnormalities associated with disease progression and accumulation of the virus itself. In the final study, I combined my passion for plant pathology and molecular techniques to explore a topic unrelated to potyviruses.</p> <p>Conclusions based on analyzing the transcripts and sRNAs of genes correlated with TuMV disease symptomology, quantifying their expression in wild-type and RNA silencing defective A. thaliana plants, and characterizing various TuMV viruses lacking RNA silencing suppressor activity will be discussed. Future directions will also be introduced.</p> | |
dc.format.mimetype | application/pdf | |
dc.identifier | archive/lib.dr.iastate.edu/etd/13049/ | |
dc.identifier.articleid | 4056 | |
dc.identifier.contextkey | 4250688 | |
dc.identifier.s3bucket | isulib-bepress-aws-west | |
dc.identifier.submissionpath | etd/13049 | |
dc.identifier.uri | https://dr.lib.iastate.edu/handle/20.500.12876/27238 | |
dc.language.iso | en | |
dc.source.bitstream | archive/lib.dr.iastate.edu/etd/13049/Campbell_iastate_0097E_13362.pdf|||Fri Jan 14 19:43:04 UTC 2022 | |
dc.subject.disciplines | Genetics | |
dc.subject.keywords | HC-Pro | |
dc.subject.keywords | Helper Component Protease | |
dc.subject.keywords | Potyvirus | |
dc.subject.keywords | qPCR | |
dc.subject.keywords | quantitative Polymerase Chain Reaction | |
dc.subject.keywords | Turnip Mosaic Virus | |
dc.title | The Turnip mosaic virus and its effects on Arabidopsis thaliana gene expression | |
dc.type | article | |
dc.type.genre | dissertation | |
dspace.entity.type | Publication | |
relation.isOrgUnitOfPublication | a26b5928-54bb-4a0b-a973-95d649d1ad83 | |
thesis.degree.level | dissertation | |
thesis.degree.name | Doctor of Philosophy |
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