Evaluation of alternative antemortem diagnostic samples for porcine reproductive and respiratory syndrome virus

dc.contributor.author Patterson, Abby
dc.contributor.author Karriker, Locke
dc.contributor.author Karriker, Locke
dc.contributor.author Evans, Richard
dc.contributor.author Yoon, Kyoung-Jin
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2019-10-23T21:06:14.000
dc.date.accessioned 2020-07-07T05:12:56Z
dc.date.available 2020-07-07T05:12:56Z
dc.date.copyright Mon Jan 01 00:00:00 UTC 2007
dc.date.issued 2007-01-01
dc.description.abstract <p><strong>Objective:</strong> To assess the diagnostic accuracy of two minimally invasive methods of blood collection and a reference method.</p> <p><strong>Materials and methods:</strong> Blood samples were collected from 30 pigs at 7 and 8 weeks of age. Fifteen pigs were then inoculated with porcine reproductive and respiratory syndrome virus (PRRSV) VR-2332 and 15 remained uninoculated. Pigs were sampled weekly for 7 weeks post inoculation (PI) using a reference sample (jugular vein sample) and two index samples (whole blood from the auricular vein collected either with a sterile polyester swab or using a capillary tube system). All samples were tested by quantitative reverse transcriptase-polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA) using established protocols. Continuous sample data for the three sampling methods were compared by analysis of the area under the receiver-operating characteristic curve.</p> <p><strong>Results:</strong> Sensitivity and specificity of qPCR testing for all samples ranged from 93% to 100% for weeks 1 through 3 PI. Results of ELISA testing depended on cutoff selection. Optimized ELISA sample:positive (S:P) ratio cutoffs for swab-sample data were significantly lower (mean S:P ratio cutoff = 0.08, SD = 0.05) than the industry standard (0.4). When the industry standard cutoff of 0.4 was utilized, swab-sample sensitivity ranged from 20.0% to 55.6% over weeks 2 through 7 PI.</p> <p><strong>Implications:</strong> Diagnosis of viremic animals using qPCR can be equivalently accomplished using any of the sampling methods. PRRS ELISA status can be determined using any of the sampling methods if an alternative S:P ratio cutoff is used.</p>
dc.description.comments <p>This article is published as Patterson, Abby R., Locke A. Karriker, Richard B. Evans, and Kyoung-Jin Yoon. "Evaluation of alternative antemortem diagnostic samples for porcine reproductive and respiratory syndrome virus." <em>Journal of Swine Health and Production</em> 15, no. 6 (2007): 339-345. Posted with permission.</p>
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dc.identifier archive/lib.dr.iastate.edu/vdpam_pubs/151/
dc.identifier.articleid 1156
dc.identifier.contextkey 15603282
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vdpam_pubs/151
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91995
dc.language.iso en, es, fr
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/151/0-2019_AASV_PermissionGrant.pdf|||Fri Jan 14 20:35:57 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/151/2007_KarrikerLocke_EvaluationAlternative.pdf|||Fri Jan 14 20:35:58 UTC 2022
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords swine
dc.subject.keywords porcine reproductive and respiratory syndrome virus
dc.subject.keywords PRRSV
dc.subject.keywords antemortem diagnosis
dc.title Evaluation of alternative antemortem diagnostic samples for porcine reproductive and respiratory syndrome virus
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication cdddf686-265a-41eb-9374-c5ff25e5120d
relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
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