In Vivo Transduction of an Stx-Encoding Phage in Ruminants

Date
2006-07-01
Authors
Cornick, Nancy
Cornick, Nancy
Helgerson, Amy
Mai, Volker
Ritchie, Jennifer
Acheson, David
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Veterinary Microbiology and Preventive Medicine
Abstract

We assessed the ability of a kanamycin-marked Stx phage to move into a commensal, ovine Escherichia coli strain in the ruminant gastrointestinal tract. Transduction was detected in 19/24 sheep tested, resulting in the recovery of 47 transductants. Subtherapeutic doses of the quinolone antibiotic enrofloxacin did not increase the rate of transduction.

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This article is from Applied and Environmental Microbiology 72 (2006): 5086, doi:10.1128/AEM.00157-06. Posted with permission.

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