Evaluation of Reverse Transcriptase 5' Nuclease Polymerase Chain Reaction assay for the Detection of Viable Heat-Injured and Resuscitated Listeria monocytogenes in Ground Pork

dc.contributor.author Boyapalle, Sandhya
dc.contributor.author Wesley, Irene
dc.contributor.author Mendonca, Aubrey
dc.date 2018-02-13T00:06:59.000
dc.date.accessioned 2020-07-02T07:01:35Z
dc.date.available 2020-07-02T07:01:35Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2003
dc.date.embargo 2012-08-15
dc.date.issued 2003-01-01
dc.description.abstract <p>An anaerobic resuscitation-enrichment system was combined with a 5' nuclease reverse transcriptase (RT) protocol for detecting Listeria monocytogenes Scott A from artificially inoculated ground pork. When irradiation-sterilized ground pork containing L. monocytogenes (~6 x 10 5 CFU/g) was heated (60 o C, 14 min), 100% of the cells were injured, as indicated by no growth on selective Modified Oxford (MOX) agar plates incubated aerobically. After resuscitation and enrichment (37°C) in anaerobic Penn State University (PSU) broth, L. monocytogenes was detected within 24 hours both by plating to MOX agar incubated in air and by a fluorogenic 5' nuclease real-time RT-PCR assay. The RT-5' nuclease polymerase chain reaction (PCR) assay targeting the hemolysin gene (hlyA) detected viable L. monocytogenes directly from the PSU within 24 hours, although a stronger signal was detected after 48 hours of resucitation. The RT-5' nuclease PCR assay bypassed the need for subsequent plating of ground pork to selective agar and thus may shorten the interval to detect low numbers of viable L. monocytogenes following heating of naturally contaminated meat.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/swinereports_2002/28/
dc.identifier.articleid 1027
dc.identifier.contextkey 3217914
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath swinereports_2002/28
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/91332
dc.language.iso en
dc.relation.ispartofseries Animal Science Research Reports
dc.relation.ispartofseries ASL R1815
dc.source.bitstream archive/lib.dr.iastate.edu/swinereports_2002/28/asl_1815.pdf|||Fri Jan 14 23:08:25 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.keywords ASL R1815
dc.title Evaluation of Reverse Transcriptase 5' Nuclease Polymerase Chain Reaction assay for the Detection of Viable Heat-Injured and Resuscitated Listeria monocytogenes in Ground Pork
dc.type article
dc.type.genre food_safety
dspace.entity.type Publication
relation.isSeriesOfPublication 7f3839b7-b833-4418-a6fa-adda2b23950a
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