Differential Expression of Ovine Innate Immune Genes by Preterm and Neonatal Lung Epithelia Infected with Respiratory Syncytial Virus
dc.contributor.author | Gallup, Jack | |
dc.contributor.author | Meyerholz, David | |
dc.contributor.author | Gallup, Jack | |
dc.contributor.author | Ackermann, Mark | |
dc.contributor.author | Lazic, Tatjana | |
dc.contributor.author | Lehmkuhl, Howard | |
dc.contributor.author | Ackermann, Mark | |
dc.contributor.department | Veterinary Pathology | |
dc.date | 2018-02-13T06:19:12.000 | |
dc.date.accessioned | 2020-07-07T05:15:44Z | |
dc.date.available | 2020-07-07T05:15:44Z | |
dc.date.copyright | Sun Jan 01 00:00:00 UTC 2006 | |
dc.date.embargo | 2013-02-25 | |
dc.date.issued | 2006-07-03 | |
dc.description.abstract | <p>Preterm infants have increased susceptibility to severe manifestations of respiratory syncytial virus (RSV) infection. The cause(s) for this age-dependent vulnerability is/are not well-defined, but alterations in innate immune products have been implicated. In sheep, RSV disease severity has similar age-dependent characteristics and sheep have several related innate molecules for study during pulmonary infection including surfactant protein A (SP-A), surfactant protein D (SP-D), sheep beta defensin 1 (SBD1), monocyte chemotactic protein 1 (MCP1), and Toll-like receptor 4 (TLR4). However, the in vivo cellular gene expression as a response to RSV infection is poorly understood. In this study, the effect of RSV infection on expression of these innate immune genes was determined for bovine RSV-infected (bRSV+ fluorescence) epithelial cells, adjacent cells lacking bRSV antigen (adjoining cells lacking fluorescence), and control cells from non-infected lung using laser capture microdissection (LCM) and real-time RT-PCR. Control lambs had increased expression of innate immune molecules in full term (term) compared to preterm epithelia with statistical significance in SBD1, SP-D, and TLR4 mRNA. Infected cells (bRSV+ fluorescent cells) had consistently higher mRNA levels of SP-A (preterm and term), MCP1 (preterm and term), and SP-D (preterm). Interestingly, bRSV- cells of infected term lambs had significantly reduced SP-D mRNA expression compared to bRSV+ and control epithelia, suggesting that RSV infected cells may regulate the adjacent epithelial SP-D expression. This study defines specific innate immune components (e.g., SBD1, SP-D, and TLR4) that have differential age-dependent expression in the airway epithelia. Furthermore, cellular bRSV infection enhanced certain innate immune components while suppressing adjacent cellular SP-D expression in term animals. These in vivo gene expression results provide a framework for future studies on age-dependent susceptibility to RSV and RSV pathogenesis.</p> | |
dc.description.comments | <p>This article is from <em>Viral Immunology </em>19, no. 2 (Summer 2006): 316–323, doi:<a href="http://dx.doi.org/10.1089/vim.2006.19.316" target="_blank">10.1089/vim.2006.19.316</a>.</p> | |
dc.format.mimetype | application/pdf | |
dc.identifier | archive/lib.dr.iastate.edu/vpath_pubs/12/ | |
dc.identifier.articleid | 1004 | |
dc.identifier.contextkey | 3782904 | |
dc.identifier.s3bucket | isulib-bepress-aws-west | |
dc.identifier.submissionpath | vpath_pubs/12 | |
dc.identifier.uri | https://dr.lib.iastate.edu/handle/20.500.12876/92434 | |
dc.language.iso | en | |
dc.source.bitstream | archive/lib.dr.iastate.edu/vpath_pubs/12/2006_Kawashima_DifferentialExpressionOvine.pdf|||Fri Jan 14 19:03:19 UTC 2022 | |
dc.source.uri | 10.1089/vim.2006.19.316 | |
dc.subject.disciplines | Veterinary Pathology and Pathobiology | |
dc.title | Differential Expression of Ovine Innate Immune Genes by Preterm and Neonatal Lung Epithelia Infected with Respiratory Syncytial Virus | |
dc.type | article | |
dc.type.genre | article | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | ef246218-d0a8-4526-8d8b-4fac40c21ae7 | |
relation.isAuthorOfPublication | 86c1ed73-b60d-48ce-8f35-449bc320a693 | |
relation.isOrgUnitOfPublication | cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f |
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