Research Notes: Linkage tests between Sp1 and Ti seed proteins

Date
1977-04-01
Authors
Orf, James
Hymowitz, Theodore
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Research Projects
Organizational Units
Series
Department
Iowa State University Digital Repository
Abstract

Polyacrylamide gel electrophoresis has been used to study the Ti and Sp1 seed proteins of the soybean. The Ti protein has been identified as the Kunitz soybean trypsin inhibitor or SBTI-A2 (Kunitz, 1945; Rackis et al., 1962; Singh, Wilson and Hadley, 1969). The three fonns of SBTI-A2 designated as Ti1, Ti2 and Ti3 are electrophoretically distinguishable from one another by their different Rf values of 0.79, 0.75 and 0.83 (Rf= mobility relative to the dye front in a 10% polyacrylamide gel anodic system using a pH 8.3 Tris-glycine buffer) respectively.

Comments
Description
Keywords
Citation
DOI
Source
Collections