Detection of extremely low concentration waterborne pathogen using a multiplexing self-referencing SERS microfluidic biosensor

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2017-02-14
Authors
Wang, Chao
Madiyar, Foram
Yu, Chenxu
Li, Jun
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Agricultural and Biosystems Engineering
Abstract

Background It is challenging to achieve ultrasensitive and selective detection of waterborne pathogens at extremely low levels (i.e., single cell/mL) using conventional methods. Even with molecular methods such as ELISA or PCR, multi-enrichment steps are needed which are labor and cost intensive. In this study, we incorporated nano-dielectrophoretic microfluidic device with Surface enhanced Raman scattering (SERS) technique to build a novel portable biosensor for easy detection and characterization of Escherichia coli O157:H7 at high sensitivity level (single cell/mL).

Results A multiplexing dual recognition SERS scheme was developed to achieve one-step target detection without the need to separate target-bound probes from unbound ones. With three different SERS-tagged molecular probes targeting different epitopes of the same pathogen being deployed simultaneously, detection of pathogen targets was achieved at single cell level with sub-species specificity that has not been reported before in single-step pathogen detection.

Conclusion The self-referencing protocol implements with a Nano-dielectrophoretic microfluidic device potentially can become an easy-to-use, field-deployable spectroscopic sensor for onsite detection of pathogenic microorganisms.

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This article is from Journal of Biological Engineering 11 (2017), doi:10.1186/s13036-017-0051-x. Posted with permission.

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Sun Jan 01 00:00:00 UTC 2017
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