Detection of extremely low concentration waterborne pathogen using a multiplexing self-referencing SERS microfluidic biosensor

dc.contributor.author Wang, Chao
dc.contributor.author Yu, Chenxu
dc.contributor.author Madiyar, Foram
dc.contributor.author Yu, Chenxu
dc.contributor.author Li, Jun
dc.contributor.department Agricultural and Biosystems Engineering
dc.date 2018-02-18T05:18:57.000
dc.date.accessioned 2020-06-29T22:42:39Z
dc.date.available 2020-06-29T22:42:39Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2017
dc.date.issued 2017-02-14
dc.description.abstract <p><strong>Background</strong> It is challenging to achieve ultrasensitive and selective detection of waterborne pathogens at extremely low levels (i.e., single cell/mL) using conventional methods. Even with molecular methods such as ELISA or PCR, multi-enrichment steps are needed which are labor and cost intensive. In this study, we incorporated nano-dielectrophoretic microfluidic device with Surface enhanced Raman scattering (SERS) technique to build a novel portable biosensor for easy detection and characterization of Escherichia coli O157:H7 at high sensitivity level (single cell/mL).</p> <p><strong>Results</strong> A multiplexing dual recognition SERS scheme was developed to achieve one-step target detection without the need to separate target-bound probes from unbound ones. With three different SERS-tagged molecular probes targeting different epitopes of the same pathogen being deployed simultaneously, detection of pathogen targets was achieved at single cell level with sub-species specificity that has not been reported before in single-step pathogen detection.</p> <p><strong>Conclusion</strong> The self-referencing protocol implements with a Nano-dielectrophoretic microfluidic device potentially can become an easy-to-use, field-deployable spectroscopic sensor for onsite detection of pathogenic microorganisms.</p>
dc.description.comments <p>This article is from <em>Journal of Biological Engineering</em> 11 (2017), doi:<a href="http://dx.doi.org/10.1186/s13036-017-0051-x">10.1186/s13036-017-0051-x</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/abe_eng_pubs/788/
dc.identifier.articleid 2075
dc.identifier.contextkey 9806801
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath abe_eng_pubs/788
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/1588
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/abe_eng_pubs/788/2017_Wang_DetectionExtremely.pdf|||Sat Jan 15 01:55:11 UTC 2022
dc.source.uri 10.1186/s13036-017-0051-x
dc.subject.disciplines Agriculture
dc.subject.disciplines Bioresource and Agricultural Engineering
dc.title Detection of extremely low concentration waterborne pathogen using a multiplexing self-referencing SERS microfluidic biosensor
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 837654ad-03e3-4004-b198-68d7756bcd27
relation.isOrgUnitOfPublication 8eb24241-0d92-4baf-ae75-08f716d30801
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