Imaging and nanodissection of individual supercoiled plasmids by atomic force microscopy
The atomic force microscope (AFM) was used to image supercooled plasmid DMA deposited on a mica surface in either a hydrated or desiccated state. Hydrated plasmid was precisely cut by the scanning tip at a location determined by the instrument operator. Small pieces of DNA (100–150 nm in length) were excised and deposited adjacent to the dissected plasmid, demonstrating that it is possible to remove and manipulate genomic DNA fragments, unresolvable by light microscopy, from defined chromosomal locations by AFM.
This article is from Nucleic Acids Research 20 (1992): 445, doi: 10.1093/nar/20.3.445. Posted with permission.