Acute Salmonella infection in swine

dc.contributor.advisor Hank Harris
dc.contributor.author Loynachan, Alan
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-08-25T04:44:49.000
dc.date.accessioned 2020-06-30T07:56:58Z
dc.date.available 2020-06-30T07:56:58Z
dc.date.copyright Sat Jan 01 00:00:00 UTC 2005
dc.date.issued 2005-01-01
dc.description.abstract <p>The objectives of this dissertation are focused on defining acute Salmonella infection in swine, and contains six chapters and two appendices. Acute Salmonella infection is defined as the ability of swine to infect alimentary and non-alimentary tissues within 3 hours following inoculation.;Chapter 1 provides a general overview of Salmonella. It includes a description of swine salmonellosis, the pig's response to Salmonella infection, methods of Salmonella detection, an overview of intervention used for the reduction of Salmonella infections, and describes acute Salmonella infection in swine.;Chapter 2 indicates that the most common swine associated Salmonella serotypes are capable of acutely infecting pigs. Pigs were inoculated with varying Salmonella serotypes, necropsied, and alimentary and non-alimentary tissues were cultured for the presence of Salmonella . In conclusion, Salmonella wildtype isolates were capable of acutely infecting pigs more so than vaccine strains.;Chapter 3 helps elucidate the mechanisms utilized by Salmonella to acutely infect swine. Varying Salmonella wildtype isolates, mutant strains, and vaccine strains were inoculated and evaluated in there abilities to acutely infect pigs. Reduction in acute Salmonella infection was seen in avirulent deletion mutants in which the cya/crp genes had been inactivated or in those strains attenuated by neutrophil passage. This study also indicated that acute infection virulence may be enhanced after continual passage of Salmonella through pigs.;Chapter 4 indicates the minimal number of Salmonella that are required to induce acute Salmonella infection. Pigs were exposed to various levels of Salmonella by either intranasal inoculation, or subjecting them to a contaminated environment. Greater than 1 X 103 Salmonellae were required to induce acute Salmonella infection.;Chapter 5 analyzes acute Salmonella infection in germfree pigs. Germfree pigs were inoculated with probiotic bacteria and subsequently challenged with Salmonella. This study established a germfree probiotic model for the reduction of acute Salmonella infection, and indicates that germfree pigs are susceptible to acute Salmonella infection.;Chapter 6 is a general conclusions chapter. Additionally, it provides suggestions for further work to advance science in the field of acute Salmonella infection in swine.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/1702/
dc.identifier.articleid 2701
dc.identifier.contextkey 6105308
dc.identifier.doi https://doi.org/10.31274/rtd-180813-13047
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/1702
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/70823
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/1702/r_3190722.pdf|||Fri Jan 14 21:13:59 UTC 2022
dc.subject.disciplines Microbiology
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.keywords Veterinary microbiology and preventive medicine
dc.subject.keywords Veterinary microbiology
dc.title Acute Salmonella infection in swine
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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