Experimental infection of conventional neonatal pigs with Clostridium difficile: A new model

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dc.contributor.author Lizer, Joshua
dc.contributor.author Ramirez, Alejandro
dc.contributor.author Madson, Darin
dc.contributor.author Schwartz, Kent
dc.contributor.author Harris, D. L.
dc.contributor.author Bosworth, Brad
dc.contributor.author Kinyon, Joann
dc.contributor.author Ramirez, Alejandro
dc.contributor.department Animal Science
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2020-01-31T16:30:06.000
dc.date.accessioned 2020-07-07T05:13:03Z
dc.date.available 2020-07-07T05:13:03Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2013
dc.date.issued 2013-01-01
dc.description.abstract <p><strong>Objective: </strong>To reproduce <em>Clostridium difficile</em>-associated disease (CDAD) using conventional pigs as an animal model.</p> <p><strong>Materials and methods: </strong>Nineteen conventional piglets, removed from the sow immediately as they were being born, were enrolled in the study. Neonatal pigs were given pooled colostrum and then transported to a research facility. At approximately 4 hours of age, 13 pigs randomly assigned to treatment groups were each orogastrically inoculated with one of two different swine-origin <em>Clostridium difficile</em> field isolates, and six control pigs were sham-inoculated. All pigs were individually housed and randomly assigned to necropsy at 24, 48, or 72 hours post infection.</p> <p><strong>Results</strong>: Commonly observed lesions and indications of CDAD, including mesocolonic edema, toxin detection, diarrhea, neutrophilic infiltration of the colonic and cecal lamina propria, and mucosal ulceration or erosion of the colon and cecum, were observed in challenged pigs.</p> <p><strong>Implications: </strong>Data generated in this study provides evidence that this has potential to be an effective challenge model for CDAD. Possible uses for this model include studies of disease pathogenesis and intervention strategies. The described model can be adapted for in vivo studies of human-origin <em>C difficile</em> strains and therapies intended for use in human medicine.</p>
dc.description.comments <p>This article is published as Lizer, Joshua T., Darin M. Madson, Kent J. Schwartz, DL Hank Harris, Brad T. Bosworth, Joann M. Kinyon, and Alejandro Ramirez. "Experimental infection of conventional neonatal pigs with Clostridium difficile: A new model." <em>Journal of Swine Health and Production</em> 21, no. 1 (2013): 22-29. Posted with permission.</p>
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dc.identifier archive/lib.dr.iastate.edu/vdpam_pubs/170/
dc.identifier.articleid 1176
dc.identifier.contextkey 16397639
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vdpam_pubs/170
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92016
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/170/2013_RamirezAlejandro_ExperimentalInfection.pdf|||Fri Jan 14 21:13:29 UTC 2022
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords swine
dc.subject.keywords Clostridium difficile
dc.subject.keywords Clostridium difficile-associated disease
dc.subject.keywords challenge model
dc.title Experimental infection of conventional neonatal pigs with Clostridium difficile: A new model
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 37ecb3cd-5b5e-4059-bc70-af2b4ff5fc1e
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