High Incidence and Endemic Spread of NDM-1-Positive Enterobacteriaceae in Henan Province, China

Qin, Shangshang
Fu, Ying
Zhang, Qijing
Zhang, Qijing
Qi, Hui
Wen, Jian
Xu, Hui
Xu, Lijuan
Zeng, Li
Tian, Hao
Rong, Lijuan
Li, Yonghong
Shan, Lihong
Xu, Hongde
Yu, Yunsong
Feng, Xianju
Liu, Hong-Min
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Veterinary Microbiology and Preventive Medicine

The emergence and spread of New Delhi metallo-β-lactamase 1 (NDM-1)-producing carbapenem-resistant Enterobacteriaceae (CRE) present an urgent threat to human health. In China, the blaNDM-1 gene has been reported mostly in Acinetobacter spp. but is rarely found in Enterobacteriaceae. Here, we report a high incidence and endemic spread of NDM-1-producing CRE in Henan Province in China. Sixteen (33.3%) of the 48 CRE isolates obtained from patients during June 2011 to July 2012 were positive for blaNDM-1, and the gene was found to be carried on plasmids of various sizes (∼55 to ∼360 kb). These plasmids were readily transferrable to recipient Escherichia coli by conjugation, conferred resistance to multiple antibiotics, and belonged to multiple replicon types. The blaNDM-1-positive CRE isolates were genetically diverse, and six new multilocus sequence typing (MLST) sequence types were linked to the carriage of NDM-1. Five of the isolates were classified as extensively drug-resistant (XDR) isolates, four of which also carried the fosA3 gene conferring resistance to fosfomycin, an alternative drug for treating infections by CRE. In each blaNDM-1-positive CRE isolate, the blaNDM-1 gene was downstream of an intact ISAba125 element and upstream of the bleMBL gene. Furthermore, gene environment analysis suggested the possible transmission of blaNDM-1-containing sequences from Acinetobacter spp. to Klebsiella pneumoniae and Klebsiella oxytoca. These findings reveal the emergence and active transmission of NDM-1-positive CRE in China and underscore the need for heightened measures to control their further spread.


This article is published as Qin, Shangshang, Ying Fu, Qijing Zhang, Hui Qi, Jian Guo Wen, Hui Xu, Lijuan Xu et al. "High Incidence and Endemic Spread of NDM-1-Positive Enterobacteriaceae in Henan Province, China." Antimicrobial Agents and Chemotherapy 58, no. 8 (2014): 4275. doi: 10.1128/AAC.02813-13. Posted with permission.