Sense–Analyze–Respond–Actuate (SARA) Paradigm: Proof of Concept System Spanning Nanoscale and Macroscale Actuation for Detection of Escherichia coli in Aqueous Media

dc.contributor.author Giacobassi, Cassie
dc.contributor.author Oliveira, Daniela
dc.contributor.author Pola, Cicero
dc.contributor.author Xiang, Dong
dc.contributor.author Tang, Yifan
dc.contributor.author Datta, Shoumen
dc.contributor.author McLamore, Eric
dc.contributor.author Gomes, Carmen
dc.contributor.department Mechanical Engineering
dc.date 2021-01-05T22:18:33.000
dc.date.accessioned 2021-02-26T03:16:13Z
dc.date.available 2021-02-26T03:16:13Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2020
dc.date.issued 2021-01-01
dc.description.abstract <p>Foodborne pathogens are a major concern for public health. We demonstrate for the first time a partially automated sensing system for rapid (~17 min), label-free impedimetric detection of Escherichia coli spp. in food samples (vegetable broth) and hydroponic media (aeroponic lettuce system) based on temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm) nanobrushes. This proof of concept (PoC) for the Sense-Analyze-Respond-Actuate (SARA) paradigm uses a biomimetic nanostructure that is analyzed and actuated with a smartphone. The bio-inspired soft material and sensing mechanism is inspired by binary symbiotic systems found in nature, where low concentrations of bacteria are captured from complex matrices by brush actuation driven by concentration gradients at the tissue surface. To mimic this natural actuation system, carbon-metal nanohybrid sensors were fabricated as the transducer layer, and coated with PNIPAAm nanobrushes. The most effective coating and actuation protocol for E. coli detection at various temperatures above/below the critical solution temperature of PNIPAAm was determined using a series of electrochemical experiments. After analyzing nanobrush actuation in stagnant media, we developed a flow through system using a series of pumps that are triggered by electrochemical events at the surface of the biosensor. SARA PoC may be viewed as a cyber-physical system that actuates nanomaterials using smartphone-based electroanalytical testing of samples. This study demonstrates thermal actuation of polymer nanobrushes to detect (sense) bacteria using a cyber-physical systems (CPS) approach. This PoC may catalyze the development of smart sensors capable of actuation at the nanoscale (stimulus-response polymer) and macroscale (non-microfluidic pumping).</p>
dc.description.comments <p>This article is published as Giacobassi, Cassie A., Daniela A. Oliveira, Cicero C. Pola, Dong Xiang, Yifan Tang, Shoumen Palit Austin Datta, Eric S. McLamore, and Carmen L. Gomes. "Sense–Analyze–Respond–Actuate (SARA) Paradigm: Proof of Concept System Spanning Nanoscale and Macroscale Actuation for Detection of Escherichia coli in Aqueous Media." <em>Actuators </em>10, no. 1 (2020): 2. DOI: <a href="https://doi.org/10.3390/act10010002" target="_blank">10.3390/act10010002</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/me_pubs/457/
dc.identifier.articleid 1459
dc.identifier.contextkey 20942481
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath me_pubs/457
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/96694
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/me_pubs/457/2021_GomesCarmen_SenseAnalyze.pdf|||Sat Jan 15 00:21:33 UTC 2022
dc.source.uri 10.3390/act10010002
dc.subject.disciplines Biology and Biomimetic Materials
dc.subject.disciplines Biomechanical Engineering
dc.subject.disciplines Nanoscience and Nanotechnology
dc.subject.disciplines Other Food Science
dc.subject.keywords Escherichia coli
dc.subject.keywords lectin
dc.subject.keywords thermo-responsive polymer
dc.subject.keywords food safety
dc.subject.keywords biosensor
dc.subject.keywords artificial reasoning tools (ART)
dc.subject.keywords sensor-analytics point solutions (SNAPS)
dc.subject.keywords Sense-Analyze-Respond-Actuate (SARA)
dc.subject.keywords percepts-environment-actuators-sensors (PEAS)
dc.subject.keywords cyber-physical systems (CPS)
dc.title Sense–Analyze–Respond–Actuate (SARA) Paradigm: Proof of Concept System Spanning Nanoscale and Macroscale Actuation for Detection of Escherichia coli in Aqueous Media
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 1bf7b7f2-528e-4d7c-a7d9-2750668775a4
relation.isOrgUnitOfPublication 6d38ab0f-8cc2-4ad3-90b1-67a60c5a6f59
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