Genetic Complementation to Identify DNA Elements That Influence Complement Resistance in Leishmania chagasi Dahlin-Laborde, Rebecca Yu, T. P. Beetham, Jeffrey
dc.contributor.department Entomology 2018-02-15T02:30:16.000 2020-06-30T02:22:57Z 2020-06-30T02:22:57Z Sat Jan 01 00:00:00 UTC 2005 2005-10-01 2005-10-01
dc.description.abstract <p>Past studies showed that <em>Leishmania</em> spp. promastigotes exhibit differential sensitivity to complement mediated lysis (CML) during development in vitro and in vivo. <em>Leishmania chagasi</em> promastigotes in cultures during logarithmic and stationary growth phases are CML-sensitive or CML-resistant when exposed to human serum, respectively, but only in cultures recently initiated with parasites from infected animals; serially passaged cultures become constitutively CML-sensitive regardless of growth phase. Building on these observations, a genetic screen was conducted to identify novel complement resistance factors of <em>L. chagasi</em>. A cosmid library containing genomic DNA was transfected into a promastigote line previously subjected to >50 serial passages. Selection with human serum for CML resistance yielded 12 transfectant clones. Cosmids isolated from 7 of these clones conferred CML resistance when transfected into an independent, high-passage promastigote culture; at 12% human serum, the mean survival of transfectants was 37% (±11.6%), and that of control transfectants was about 1%. Inserts within the 7 cosmids were unique. Determination of the complete DNA sequence for 1 cosmid indicated that its 32-kilobase insert was 89% identical (overall) to a 31-kilobase region of <em>Leishmania major</em> chromosome 36, which is predicted to encode 6 genes, all of which encode hypothetical proteins.</p>
dc.description.comments <p>This article is from <em>Journal of Parasitology</em> 91 (2005): 1058, doi:<a href="" target="_blank">10.1645/GE-477R.1</a>. Posted with permission.</p>
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dc.identifier archive/
dc.identifier.articleid 1267
dc.identifier.contextkey 6226324
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ent_pubs/265
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 23:03:14 UTC 2022
dc.source.uri 10.1645/GE-477R.1
dc.subject.disciplines Entomology
dc.subject.disciplines Genetics
dc.subject.disciplines Genomics
dc.subject.disciplines Parasitology
dc.subject.keywords cosmids
dc.subject.keywords complement system proteins
dc.subject.keywords genetic vectors
dc.subject.keywords restriction mapping
dc.title Genetic Complementation to Identify DNA Elements That Influence Complement Resistance in Leishmania chagasi
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 1505fb83-30dc-4128-b6d9-29b02ed91f6b
relation.isOrgUnitOfPublication f47c8cad-50be-4fb0-8870-902ff536748c
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