ZmbZIP54 and ZmFDX5 cooperatively regulate maize seedling tolerance to lead by mediating ZmPRP1 transcription

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2022-10-20
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Hou, Fengxia
Zhang, Na
Ma, Langlang
An, Lijun
Zhou, Xun
Zou, Chaoying
Yang, Cong
Pan, Guangtang
Shen, Yaou
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© 2022 Elsevier B.V.
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Agronomy

The Department of Agronomy seeks to teach the study of the farm-field, its crops, and its science and management. It originally consisted of three sub-departments to do this: Soils, Farm-Crops, and Agricultural Engineering (which became its own department in 1907). Today, the department teaches crop sciences and breeding, soil sciences, meteorology, agroecology, and biotechnology.

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The Department of Agronomy was formed in 1902. From 1917 to 1935 it was known as the Department of Farm Crops and Soils.

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1902–present

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  • Department of Farm Crops and Soils (1917–1935)

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Abstract
Extensive lead (Pb) accumulation in plants exerts toxic effects on plant growth and development and enters the human food chain. Combining linkage mapping, transcriptome analysis, and association studies, we cloned the ZmbZIP54 transcription factor, which confers maize tolerance to Pb. Combined overexpression and knockdown confirmed that ZmbZIP54 mitigates Pb toxicity in maize by alleviating Pb absorption into the roots. Yeast one-hybrid and dual-luciferase assays revealed that ZmbZIP54 binds to the ZmPRP1 promoter and promotes its transcription. Yeast two-hybrid and bimolecular fluorescence complementation assays indicated that ZmFdx5 interacts with ZmbZIP54 in the nucleus. ZmFdx5 acts as a switch that controls the regulation of ZmPRP1 expression by ZmbZIP54 when maize encounters Pb stress. Furthermore, we revealed that variation in the 5′-UTR of ZmbZIP54 affects its expression level under Pb stress and contributes to the difference in Pb tolerance among maize lines. Finally, we proposed a model to summarize the role of ZmbZIP54 in Pb tolerance, which involves the cooperative effect of ZmbZIP54 and ZmFdx5 on the ZmPRP1 transcription in maize response to Pb. This study provides novel insights into the development of Pb-tolerant maize varieties and bioremediation of Pb-contaminated soils.
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This is a manuscript of an article published as Hou, Fengxia, Na Zhang, Langlang Ma, Lijun An, Xun Zhou, Chaoying Zou, Cong Yang, Guangtang Pan, Thomas Lübberstedt, and Yaou Shen. "ZmbZIP54 and ZmFDX5 cooperatively regulate maize seedling tolerance to lead by mediating ZmPRP1 transcription." International Journal of Biological Macromolecules (2022). doi:10.1016/j.ijbiomac.2022.10.151. Posted with permission.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
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