High-Spatial and High-Mass Resolution Imaging of Surface Metabolites of Arabidopsis thaliana by Laser Desorption-Ionization Mass Spectrometry Using Colloidal Silver

dc.contributor.author Jun, Ji Hyun
dc.contributor.author Song, Zhihong
dc.contributor.author Liu, Zhenjiu
dc.contributor.author Lee, Young Jin
dc.contributor.author Nikolau, Basil
dc.contributor.author Yeung, Edward
dc.contributor.department Ames National Laboratory
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.contributor.department Chemistry
dc.date 2018-02-17T09:48:22.000
dc.date.accessioned 2020-06-30T01:23:42Z
dc.date.available 2020-06-30T01:23:42Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2010
dc.date.issued 2010-03-17
dc.description.abstract <p>High-spatial resolution and high-mass resolution techniques are developed and adopted for the mass spectrometric imaging of epicuticular lipids on the surface of <em>Arabidopsis thaliana</em>. Single cell level spatial resolution of ∼12 μm was achieved by reducing the laser beam size by using an optical fiber with 25 μm core diameter in a vacuum matrix-assisted laser desorption ionization-linear ion trap (vMALDI-LTQ) mass spectrometer and improved matrix application using an oscillating capillary nebulizer. Fine chemical images of a whole flower were visualized in this high spatial resolution showing substructure of an anther and single pollen grains at the stigma and anthers. The LTQ-Orbitrap with a MALDI ion source was adopted to achieve MS imaging in high mass resolution. Specifically, isobaric silver ion adducts of C29 alkane (<em>m</em>/<em>z</em> 515.3741) and C28 aldehyde (<em>m</em>/<em>z</em> 515.3377), indistinguishable in low-resolution LTQ, can now be clearly distinguished and their chemical images could be separately constructed. In the application to roots, the high spatial resolution allowed molecular MS imaging of secondary roots and the high mass resolution allowed direct identification of lipid metabolites on root surfaces.</p>
dc.description.comments <p>Reprinted (adapted) with permission from <em>Analytical Chemistry</em> 82 (2010): 3255, doi: <a href="http://dx.doi.org/10.1021/ac902990p" target="_blank">10.1021/ac902990p</a>. Copyright 2010 American Chemical Society.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/chem_pubs/893/
dc.identifier.articleid 1899
dc.identifier.contextkey 7969450
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath chem_pubs/893
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/15393
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/chem_pubs/893/0-2010_LeeYJ_HighSpatialHigh.pdf|||Sat Jan 15 02:19:17 UTC 2022
dc.source.bitstream archive/lib.dr.iastate.edu/chem_pubs/893/2010_LeeYJ_HighSpatialHigh.pdf|||Sat Jan 15 02:19:18 UTC 2022
dc.source.uri 10.1021/ac902990p
dc.subject.disciplines Analytical Chemistry
dc.subject.disciplines Chemistry
dc.title High-Spatial and High-Mass Resolution Imaging of Surface Metabolites of Arabidopsis thaliana by Laser Desorption-Ionization Mass Spectrometry Using Colloidal Silver
dc.type article
dc.type.genre article
dspace.entity.type Publication
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