FOXO3 Expression and Function in the Pig Oocyte and Embryo

Thumbnail Image
Date
2013-01-01
Authors
Yang, Caixia
Wright, Elane
Hale, Benjamin
Ross, Jason
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Person
Keating, Aileen
Professor
Research Projects
Organizational Units
Journal Issue
Is Version Of
Versions
Series
Department
Abstract

Forkhead box O3 (FOXO3), a member of the FOXO subfamily of Forkhead transcription factors, has been shown to play critical roles in apoptosis, oxidative stress, cell cycle and DNA repair. The objective of this study was to characterize FOXO3 expression and its function during oocyte maturation and early embryo development in the pig.We found: (1) FOXO3 is dynamically expressed at both mRNA and protein level in the maturing oocyte and early in vitro fertilized embryos. (2) FOXO3 protein is localized in the cytoplasm of pig maturing oocytes. (3) Co-culture with Doxorubicin (DOX, 2 µM) significantly altered the total FOXO3 protein level during in vitro maturation. (4) in vitromaturation with DOX (2µM) numerically increased maturation rate, but significantly decreased embryo development to the blastocyst stage after oocyte parthenogenetic activation. Our work provides useful data for functional study of FOXO3 protein in female gametogenesis and the potential impact on developing pig embryos, which could benefit animal reproductive health and provide foundational knowledge for improving swine reproductive efficiency.

Comments
Description
Keywords
Citation
Source
Keywords
Copyright
Tue Jan 01 00:00:00 UTC 2013
Collections