Membrane trafficking in resistance gene-mediated defense against the barley powdery mildew fungus

dc.contributor.advisor Roger P. Wise
dc.contributor.author Surana, Priyanka
dc.contributor.department Plant Pathology and Microbiology
dc.date 2019-09-18T20:14:19.000
dc.date.accessioned 2020-06-30T03:17:38Z
dc.date.available 2020-06-30T03:17:38Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2017
dc.date.embargo 2019-06-23
dc.date.issued 2017-01-01
dc.description.abstract <p>Blumeria graminis f. sp. hordei (Bgh) is the causal agent of powdery mildew on barley. Bgh secretes over 700 proteins that may function to modify host processes for colonization. Differential expression analysis of Bgh genes encoding secreted proteins in an isogenic panel of host immune signaling mutants, including a novel mutation Rar3 (Required for Mla6 Resistance3), identified fungal membrane trafficking genes. Infection phenotyping and kinetics show that immune compromised mutants have similar fungal growth, thereby, attributing any differences observed in expression to mutation rather than development. Thus, we hypothesize that powdery mildew genes may be targeting the host membrane trafficking pathway to both internalize effector proteins and suppress plant defense signal relay. Next, to address the modulation of host membrane trafficking genes, we utilized differential expression analysis. This identified over 100 genes in all immune-compromised mutants, including ADP Ribosylation Factor 1 (ARF1) during host penetration by Bgh. ARF1 is a GTP-binding protein, involved in protein trafficking, ER-to-Golgi transport and Golgi-derived transport to the plasma membrane. Additionally, we utilized an expression Quantitative Trait Locus (eQTL) approach to focus on genes associated with Mla. This led to the discovery of 3,296 genes, including those in membrane trafficking pathways, that are directly or indirectly regulated by the Mla region (1HS). Taken together, these results corroborate the pivotal role membrane trafficking plays in R gene-mediated defense against powdery mildew.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/17330/
dc.identifier.articleid 8337
dc.identifier.contextkey 15016708
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/17330
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/31513
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/17330/Surana_iastate_0097E_16021.pdf|||Fri Jan 14 21:20:50 UTC 2022
dc.subject.disciplines Agriculture
dc.subject.disciplines Bioinformatics
dc.subject.disciplines Genetics
dc.subject.disciplines Plant Pathology
dc.subject.keywords barley plant
dc.subject.keywords eQTL
dc.subject.keywords host pathogen interactions
dc.subject.keywords powdery mildew fungus
dc.subject.keywords R genes
dc.subject.keywords RNA Seq
dc.title Membrane trafficking in resistance gene-mediated defense against the barley powdery mildew fungus
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication a26b5928-54bb-4a0b-a973-95d649d1ad83
thesis.degree.discipline Bioinformatics and Computational Biology
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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