Novel technologies in doubled haploid line development

Date
2017-01-01
Authors
Ren, Jiaojiao
Wu, Penghao
Lubberstedt, Thomas
Trampe, Benjamin
Tian, Xiaolong
Chen, Shaojiang
Lubberstedt, Thomas
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Agronomy
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Agronomy
Abstract

haploid inducer line can be transferred (DH) technology can not only shorten the breeding process but also increase genetic gain. Haploid induction and subsequent genome doubling are the two main steps required for DH technology. Haploids have been generated through the culture of immature male and female gametophytes, and through inter- and intraspecific via chromosome elimination. Here, we focus on haploidization via chromosome elimination, especially the recent advances in centromere-mediated haploidization. Once haploids have been induced, genome doubling is needed to produce DH lines. This study has proposed a new strategy to improve haploid genome doubling by combing haploids and minichromosome technology. With the progress in haploid induction and genome doubling methods, DH technology can facilitate reverse breeding, cytoplasmic male sterile (CMS) line production, gene stacking and a variety of other genetic analysis.

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This article is published as Ren, Jiaojiao, Penghao Wu, Benjamin Trampe, Xiaolong Tian, Shaojiang Chen, and Thomas Lübberstedt. "Novel technologies in doubled haploid line development." Plant Biotechnology Journal (2017). 10.1111/pbi.12805. Posted with permission.

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