Mycobacterium bovis infection in cattle in Morocco: preparation and evaluation of chemical extracts for use in detection of immune responses

dc.contributor.advisor Charles O. Thoen Berrada, Jaouad
dc.contributor.department Veterinary Microbiology and Preventive Medicine 2018-08-23T05:10:51.000 2020-06-30T07:04:32Z 2020-06-30T07:04:32Z Fri Jan 01 00:00:00 UTC 1993 1993
dc.description.abstract <p>Sodium deoxycholate (DOC) was utilized to extract immunologically-active cell wall components of virulent M. bovis and M. avium, isolated from cattle in Morocco and M. fortuitum as well as related organisms e.g., N. asteroides and R. equi. The extracts from M. bovis, N. asteroides, M. fortuitum, and M. avium, were fractionated by size exclusion chromatography using Sephadex S-200. Two fractions (1 and 2) were obtained at absorbance of 280 nm. Fraction 1 of M. bovis, M. avium, M. fortuitum and of N. asteroides and nonfractionated DOC extract of R. equi elicited delayed-type hypersensitivity responses in homologously or heterologously sensitized guinea-pigs. Significant enzyme-linked immunosorbent assay values were obtained using sera from cattle 4 weeks after sensitization with homologous bacterial preparations (p < 0.01). Significant ELISA cross-reactions were observed in M. bovis or M. avium sensitized calves, using heterologous antigens;Mycobacteriologic examinations conducted on tissues collected from 246 cows in 5 major abattoirs of Morocco, revealed that M. bovis was isolated from 29.67% of the cattle examined. Mycobacteria other than M. bovis (MOTT) were isolated from tissues of 23 carcasses (9.34%). Three had lesions compatible with tuberculosis; M. bovis was not isolated;An indirect enzyme-linked immunosorbent assay using M. bovis PPD or M. bovis DOC fraction 1 as antigens was used for detecting antibodies in sera of cattle from which M. bovis was isolated. The sensitivity for M. bovis PPD was 76.71% and for DOC extract of M. bovis was 79.45%; the specificity was of 90% and 92%, respectively. These values are similar to those achieved by the tuberculin skin test (71);Investigations conducted on five selected herds in Morocco using the cervical intradermal tuberculin test and ELISA revealed that cattle in three herds were tuberculous. The comparative cervical tuberculin test using M. bovis and M. avium PPD's at equal concentration was conducted within 24 hours of a positive or suspect skin response; no reactors were identified in the remaining two herds;Combination of ELISA and tuberculin skin test conducted on animals from tuberculous herds was found to be more sensitive than either test conducted alone.</p>
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dc.identifier.articleid 11402
dc.identifier.contextkey 6404610
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dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 18:20:35 UTC 2022
dc.subject.disciplines Microbiology
dc.subject.disciplines Veterinary Medicine
dc.subject.keywords Microbiology
dc.subject.keywords immunology and preventive medicine
dc.subject.keywords Veterinary microbiology
dc.title Mycobacterium bovis infection in cattle in Morocco: preparation and evaluation of chemical extracts for use in detection of immune responses
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83 dissertation Doctor of Philosophy
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