Enumeration of Escherichia coli O157:H7 from ground beef using a re-circulating immuno-magnetic bead separation

dc.contributor.author Jackson, Armitra
dc.contributor.department Animal Science
dc.date 2020-06-20T02:34:29.000
dc.date.accessioned 2020-06-30T08:14:38Z
dc.date.available 2020-06-30T08:14:38Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2006
dc.date.issued 2006-01-01
dc.description.abstract <p>The ability of magnetic immunocapture to enumerate Escherichia coli 0157.H7 in ground beef was investigated. Ground beef was inoculated with a known quantity of a five strain mixed culture of E. coli 0157.H7, and the population subsequently enumerated by re-circulating the ground beef sample with immuno-magnetic beads. Trypsin and Polyoxyethylene Sorbitan Monolaurate (tween 20) were added to the ground beef sample and allowed to incubate for one hour to achieve a higher recovery of the target bacteria. There was statistical difference (P<0.05) between the known population inoculated into the ground beef and the populations enumerated in the ground beef by the immunocapture method. The regression model showed that there is a linear relationship between the two groups. This model proves that the Pathatrix System can be used for the enumeration of E. coli 0157.H7 in ground beef.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/19296/
dc.identifier.articleid 20295
dc.identifier.contextkey 18174272
dc.identifier.doi https://doi.org/10.31274/rtd-20200618-8
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/19296
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/73290
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/19296/Jackson_ISU_2006_J33.pdf|||Fri Jan 14 21:54:11 UTC 2022
dc.subject.keywords Animal science
dc.subject.keywords Meat science
dc.title Enumeration of Escherichia coli O157:H7 from ground beef using a re-circulating immuno-magnetic bead separation
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
thesis.degree.discipline Meat Science
thesis.degree.level thesis
thesis.degree.name Master of Science
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