Agrobacterium tumefaciens-Mediated Transformation of Maize Embryos Using a Standard Binary Vector System Frame, Bronwyn Nettleton, Dan Shou, Huixia Chikwamba, Rachel Zhang, Zhanyuan Xiang, Chengbin Fonger, Tina Pegg, Sue Ellen Li, Baochun Nettleton, Dan Pei, Deqing Wang, Kan
dc.contributor.department Statistics
dc.contributor.department Agronomy 2019-09-01T09:17:22.000 2020-07-02T06:57:18Z 2020-07-02T06:57:18Z Tue Jan 01 00:00:00 UTC 2002 2002-05-01
dc.description.abstract <p>We have achieved routine transformation of maize (<em>Zea mays</em>) using an <em>Agrobacterium tumefaciens</em>standard binary (non-super binary) vector system. Immature zygotic embryos of the hybrid line Hi II were infected with <em>A. tumefaciens</em> strain EHA101 harboring a standard binary vector and cocultivated in the presence of 400 mg L−1 l-cysteine. Inclusion of l-cysteine in cocultivation medium lead to an improvement in transient β-glucuronidase expression observed in targeted cells and a significant increase in stable transformation efficiency, but was associated with a decrease in embryo response after cocultivation. The average stable transformation efficiency (no. of bialaphos-resistant events recovered per 100 embryos infected) of the present protocol was 5.5%. Southern-blot and progeny analyses confirmed the integration, expression, and inheritance of the <em>bar</em> and<em>gus</em>transgenes in R0, R1, and R2 generations of transgenic events. To our knowledge, this represents the first report in which fertile, stable transgenic maize has been routinely produced using an <em>A. tumefaciens</em>standard binary vector system.</p>
dc.description.comments <p>This article is published as Frame, Bronwyn R., Huixia Shou, Rachel K. Chikwamba, Zhanyuan Zhang, Chengbin Xiang, Tina M. Fonger, Sue Ellen K. Pegg et al. "Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system." <em>Plant physiology</em> 129, no. 1 (2002): 13-22. doi: <a href="" target="_blank">10.1104/pp.000653</a>. Posted with permission.</p>
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dc.identifier archive/
dc.identifier.articleid 1239
dc.identifier.contextkey 14902546
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dc.identifier.submissionpath stat_las_pubs/226
dc.language.iso en
dc.source.bitstream archive/|||Fri Jan 14 22:43:59 UTC 2022
dc.source.uri 10.1104/pp.000653
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Applied Statistics
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.disciplines Plant Pathology
dc.subject.disciplines Statistical Methodology
dc.title Agrobacterium tumefaciens-Mediated Transformation of Maize Embryos Using a Standard Binary Vector System
dc.type article
dc.type.genre article
dspace.entity.type Publication
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