Fast detection of pathogenic bacteria by PCR using premixed primer sets

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2001-01-01
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Schönfels, Cristoph
Gabert, Jörg
Blechschmidt, Nicole
Grimmecke, Hans-Dieter
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International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork
Iowa State University Conferences and Symposia

The SafePork conference series began in 1996 to bring together international researchers, industry, and government agencies to discuss current Salmonella research and identify research needs pertaining to both pig and pork production. In subsequent years topics of research presented at these conferences expanded to include other chemical and biological hazards to pig and pork production.

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Amplification of DNA is the most sensitive tool for the straight detection of pathogenic organisms. Particularly, bacterial pathogens can be recognized easily and rapidly if specific primers are available for PCR. One of the crucial efforts of Biotype Diagnostic GmbH is the development and production of ready-to-use premixed PCR tests for diagnostics of bacterial diseases in human and veterinary medicine. Special focus is directed to Mycoplasma gallisepticum, Mycoplasma synoviae, to gram-positive (Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Mycobacterium avium subspecies paratuberculosis) and gram-negative (Actinobacillus pleuropneumoniae, Bordetella bronchiseptica, Salmonella enterica) bacteria.

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