Leukocyte transcriptome from chickens infected with avian pathogenic Escherichia coli identifies pathways associated with resistance

dc.contributor.author Nolan, Lisa
dc.contributor.author Sandford, Erin
dc.contributor.author Orr, Megan
dc.contributor.author Shelby, Mandy
dc.contributor.author Lamont, Susan
dc.contributor.author Li, Xianyao
dc.contributor.author Zhou, Huaijun
dc.contributor.author Johnson, Timothy
dc.contributor.author Kariyawasam, Subhashinie
dc.contributor.author Liu, Peng
dc.contributor.author Nolan, Lisa
dc.contributor.author Lamont, Susan
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.date 2018-02-13T10:38:08.000
dc.date.accessioned 2020-07-07T05:14:24Z
dc.date.available 2020-07-07T05:14:24Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2012
dc.date.embargo 2013-05-06
dc.date.issued 2012-01-01
dc.description.abstract <p>Avian pathogenic <em>Escherichia coli</em> (APEC) causes colibacillosis, which is responsible for morbidity and mortality in chickens. Gene expression patterns have previously been demonstrated to differ between chicken populations that are resistant vs. susceptible to bacterial infection, but little is currently known about gene expression response to APEC. Increased understanding of gene expression patterns associated with resistance will facilitate genetic selection to increase resistance to APEC. Male broiler chicks were vaccinated at 2 weeks of age and challenged with APEC at 4 weeks of age. Peripheral blood leukocytes were collected at 1 and 5 day post-infection. Lesions on the liver, pericardium, and air sacs were used to assign a mild or severe pathology status to non-vaccinated, challenged chicks. Ten treatment groups were therefore generated with <em>a priori</em> factors of vaccination, challenge, day post-infection, and the <em>a posteriori</em> factor of pathology status. Global transcriptomic response was evaluated using the Agilent 44K chicken microarray. APEC infection resulted in more up-regulation than down-regulation of differentially expressed genes. Immune response and metabolic processes were enriched with differentially expressed genes. Although vaccination significantly reduced lesions in challenged bird, there was no detectable effect of vaccination on gene expression. This study investigated the transcriptomic differences in host responses associated with mild vs. severe pathology, in addition to the effects of vaccination and challenge, thus revealing genes and networks associated with response to APEC and providing a foundation for future studies on, and genetic selection for, genetic resistance to APEC.</p>
dc.description.comments <p>This article is from <em>Results in Immunology</em> 2 (2012): 44–53, doi:<a href="http://dx.doi.org/10.1016/j.rinim.2012.02.003" id="x-ddDoi" target="_blank">10.1016/j.rinim.2012.02.003</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vmpm_pubs/12/
dc.identifier.articleid 1020
dc.identifier.contextkey 4110232
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vmpm_pubs/12
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92221
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vmpm_pubs/12/2012_SandfordEE_LeukocyteTranscriptomeChickens.pdf|||Fri Jan 14 19:03:23 UTC 2022
dc.source.uri 10.1016/j.rinim.2012.02.003
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Biostatistics
dc.subject.disciplines Poultry or Avian Science
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords Animal Science
dc.subject.keywords Statistics
dc.subject.keywords Avian pathogenic Escherichia coli
dc.subject.keywords Leukocytes
dc.subject.keywords Microarray
dc.subject.keywords Transcriptome
dc.subject.keywords Immune response
dc.subject.keywords Chicken
dc.title Leukocyte transcriptome from chickens infected with avian pathogenic Escherichia coli identifies pathways associated with resistance
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 9e7506b4-e945-47cf-9195-e814dac6c9fd
relation.isAuthorOfPublication 5dee3d24-aa7a-4fe1-abf6-f0bb615bfe24
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83
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