Spectral hole burning studies of Photosystem 1

dc.contributor.advisor Gerald J. Small
dc.contributor.author Gillie, J. Kevin
dc.contributor.department Chemistry
dc.date 2018-08-15T04:12:04.000
dc.date.accessioned 2020-07-02T06:10:09Z
dc.date.available 2020-07-02T06:10:09Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 1989
dc.date.issued 1989
dc.description.abstract <p>Persistent spectral hole burning is applied to the reaction center, P700, and the light harvesting chlorophyll protein complexes of Photosystem I. A theory for solid state spectral hole burning is developed that is valid for arbitrarily strong linear electron-phonon coupling within the Condon approximation. Persistent photochemical hole burning of the reaction center P700, reveals that a broad (~300 cm[superscript] -1) hole can be burned into the adsorption profile. The hole profile and its maximum position and intensity dependence on burn wavelength are adequately fit by the electron-phonon coupling theory. The results indicate that the absorption and hole profile are dominated by phonon transitions with a Huang-Rhys factor of ~8. A dimer structure for P700 is supported. The similarities to the primary electron donor states of other reaction centers are examined;Nonphotochemical hole burning spectra for the Q[subscript] y transitions associated with the light harvesting antenna complex of Photosystem I are presented. The frequencies and Franck-Condon factors are determined for 51 chlorophyll a and 12 chlorophyll b intramolecular modes. The linear electron-vibration coupling for all modes is very weak with the maximum Franck-Condon factor observed being ~0.04. The linear electron-phonon coupling for protein modes of mean frequency 22 cm[superscript] -1 is weak with Huang-Rhys factor of 0.8. The electron-phonon coupling of the antenna system is compared with that for P700. The intramolecular modes, phonon frequencies, and Franck-Condon factors are used with multiphonon excitation transport theories to analyze the available temperature-dependent data on the kinetics of transport within the core antenna complex. Phonons (not intramolecular modes) mediate excitation transport within the antenna and from antenna to reaction center. The results also indicate that a subunit or cluster model for the antenna provide a more accurate picture than the regular array model for excitation transport.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/8935/
dc.identifier.articleid 9934
dc.identifier.contextkey 6344837
dc.identifier.doi https://doi.org/10.31274/rtd-180813-11933
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/8935
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/81978
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/8935/r_8920132.pdf|||Sat Jan 15 02:19:19 UTC 2022
dc.subject.disciplines Biophysics
dc.subject.disciplines Physical Chemistry
dc.subject.keywords Chemistry
dc.subject.keywords Physical chemistry
dc.title Spectral hole burning studies of Photosystem 1
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 42864f6e-7a3d-4be3-8b5a-0ae3c3830a11
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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