Use of Bromodeoxyuridine Immunohistochemistry for the Study of Cytogenesis and Neurochemical and Cellular Differentiation in the Marsupial Brain and Retina

Date
1997
Authors
Iqbal, Javed
Sakaguchi, Donald
Jensen, Chris
Jacobson, Carol
Sakaguchi, Donald
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Altmetrics
Authors
Research Projects
Organizational Units
Zoology and Genetics
Organizational Unit
Veterinary Anatomy
Organizational Unit
Neuroscience
Organizational Unit
Journal Issue
Series
Department
Zoology and GeneticsVeterinary AnatomyNeuroscience
Abstract

Determining the birthdate of neurons and glia in relation lO the time period of neurochemical 1.nd cellular differentiation are necessary for understanding early developmental events in the central nerv.>us system (CNS). Bromodeoxyuridine (BrdU). a thymidine analog has recently become an alternative to the tritiated thymidine autoradiography for the study of cytogenesis in the CNS. Our laboratory utilizes the Brazilian opossum, Monodelphis domestica to study the ontogeny of neuropeptide systems and the development of the visual system in the mammalian brain. Opossum pups are born in an extremely immature state before neurogenesis and morphogenesis are completed and provide an excellent experimental model for developmental studies. To this end, we have developed single, double. and triple label BrctU immunohistochemical procedures for simultaneous study of cytogenesis and cellular differentiation in the opossum brain and retina. These methods are simple, rapid, and very effective and could be widely applied for understanding developmental events in the CNS.

Comments

This article is from Pakistan Veterinary Journal 17 (1997): 139. Posted with permission.

Description
Keywords
Citation
DOI
Source
Collections