Evaluating PHA Productivity of Bioengineered Rhodosprillum rubrum

dc.contributor.author Jin, Huanan
dc.contributor.author Nikolau, Basil
dc.contributor.department Biochemistry, Biophysics and Molecular Biology
dc.contributor.department Center for Biorenewable Chemicals
dc.date 2018-02-17T11:09:40.000
dc.date.accessioned 2020-06-29T23:47:14Z
dc.date.available 2020-06-29T23:47:14Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.issued 2014-01-01
dc.description.abstract <p>This study explored the potential of using <em>Rhodosprillum rubrum</em> as the biological vehicle to convert chemically simple carbon precursors to a value-added bio-based product, the biopolymer PHA. <em>R. rubrum</em> strains were bioengineered to overexpress individually or in various combinations, six PHA biosynthetic genes (<em>phaC1</em>, <em>phaA</em>, <em>phaB</em>, <em>phaC2</em>, <em>phaC3, and phaJ</em>), and the resulting nine over-expressing strains were evaluated to assess the effect on PHA content, and the effect on growth. These experiments were designed to genetically evaluate: 1) the role of each apparently redundant PHA polymerase in determining PHA productivity; 2) identify the key gene(s) within the <em>pha</em> biosynthetic operon that determines PHA productivity; and 3) the role of <em>phaJ</em> to support PHA productivity. The result of overexpressing each PHA polymerase-encoding gene indicates that <em>phaC1</em> and <em>phaC2</em> are significant contributors to PHA productivity, whereas <em>phaC3</em> has little effect. Similarly, over-expressing individually or in combination the three PHA biosynthesis genes located in the <em>pha</em> operon indicates that <em>phaB</em> is the key determinant of PHA productivity. Finally, analogous experiments indicate that <em>phaJ</em> does not contribute significantly to PHA productivity. These bioengineering strains achieved PHA productivity of up to 30% of dry biomass, which is approximately 2.5-fold higher than the non-engineered control strain, indicating the feasibility of using this approach to produce value added bio-based products.</p>
dc.description.comments <p>This is an article from PLoS ONE 9 (2014): 1, doi:<a href="http://dx.doi.org/10.1371/journal.pone.0096621%20" target="_blank">10.1371/journal.pone.0096621</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/bbmb_ag_pubs/53/
dc.identifier.articleid 1063
dc.identifier.contextkey 8046156
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath bbmb_ag_pubs/53
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/10785
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/bbmb_ag_pubs/53/2014_Nikolau_EvaluatingPHA.pdf|||Sat Jan 15 00:50:13 UTC 2022
dc.source.uri 10.1371/journal.pone.0096621
dc.subject.disciplines Biochemistry, Biophysics, and Structural Biology
dc.subject.disciplines Other Chemistry
dc.title Evaluating PHA Productivity of Bioengineered Rhodosprillum rubrum
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 73b33082-7300-4c56-8019-d5e13b9898de
relation.isOrgUnitOfPublication c70f85ae-e0cd-4dce-96b5-4388aac08b3f
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