Soybean proteins GmTic110 and GmPsbP are crucial for chloroplast development and function

Date
2016-11-01
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Sandhu, Devinder
Atkinson, Taylor
Goggi, A. Susana
Noll, Andrea
Johnson, Callie
Espinosa, Katherine
Boelter, Jessica
Abel, Stephanie
Dhatt, Balpreet
Barta, Terese
Singsaas, Eric
Sepsenwol, Sol
Goggi, A. Susana
Palmer, Reid
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Agronomy
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Agronomy
Abstract

We have identified a viable-yellow and a lethal-yellow chlorophyll-deficient mutant in soybean. Segregation patterns suggested single-gene recessive inheritance for each mutant. The viable- and lethal-yellow plants showed significant reduction of chlorophyll aand b. Photochemical energy conversion efficiency and photochemical reflectance index were reduced in the viable-yellow plants relative to the wildtype, whereas the lethal-yellow plants showed no electron transport activity. The viable-yellow plants displayed reduced thylakoid stacking, while the lethal-yellow plants exhibited failure of proplastid differentiation into normal chloroplasts with grana. Genetic analysis revealed recessive epistatic interaction between the viable- and the lethal-yellow genes. The viable-yellow gene was mapped to a 58 kb region on chromosome 2 that contained seven predicted genes. A frame shift mutation, due to a single base deletion in Glyma.02g233700, resulted in an early stop codon. Glyma.02g233700 encodes a translocon in the inner membrane of chloroplast (GmTic110) that plays a critical role in plastid biogenesis. The lethal-yellow gene was mapped to an 83 kb region on chromosome 3 that contained 13 predicted genes. Based on the annotated functions, we sequenced three potential candidate genes. A single base insertion in the second exon of Glyma.03G230300 resulted in a truncated protein. Glyma.03G230300 encodes for GmPsbP, an extrinsic protein of Photosystem II that is critical for oxygen evolution during photosynthesis. GmTic110 and GmPsbP displayed highly reduced expression in the viable- and lethal-yellow mutants, respectively. The yellow phenotypes in the viable- and lethal-yellow mutants were due to the loss of function of GmTic110 or GmPsbP resulting in photooxidative stress.

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This article is published as Sandhu, Devinder, Taylor Atkinson, Andrea Noll, Callie Johnson, Katherine Espinosa, Jessica Boelter, Stephanie Abel et al. "Soybean proteins GmTic110 and GmPsbP are crucial for chloroplast development and function." Plant Science 252 (2016): 76-87. doi: 10.1016/j.plantsci.2016.07.006.

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