Soybean proteins GmTic110 and GmPsbP are crucial for chloroplast development and function

dc.contributor.author Sandhu, Devinder
dc.contributor.author Atkinson, Taylor
dc.contributor.author Noll, Andrea
dc.contributor.author Johnson, Callie
dc.contributor.author Espinosa, Katherine
dc.contributor.author Boelter, Jessica
dc.contributor.author Abel, Stephanie
dc.contributor.author Dhatt, Balpreet
dc.contributor.author Barta, Terese
dc.contributor.author Singsaas, Eric
dc.contributor.author Sepsenwol, Sol
dc.contributor.author Goggi, A. Susana
dc.contributor.author Palmer, Reid
dc.contributor.department Agronomy
dc.date 2018-06-13T16:07:14.000
dc.date.accessioned 2020-06-29T23:05:38Z
dc.date.available 2020-06-29T23:05:38Z
dc.date.issued 2016-11-01
dc.description.abstract <p>We have identified a viable-yellow and a lethal-yellow chlorophyll-deficient mutant in soybean. Segregation patterns suggested single-gene recessive inheritance for each mutant. The viable- and lethal-yellow plants showed significant reduction of chlorophyll <em>a</em>and <em>b</em>. Photochemical energy conversion efficiency and photochemical reflectance index were reduced in the viable-yellow plants relative to the wildtype, whereas the lethal-yellow plants showed no electron transport activity. The viable-yellow plants displayed reduced thylakoid stacking, while the lethal-yellow plants exhibited failure of proplastid differentiation into normal chloroplasts with grana. Genetic analysis revealed recessive epistatic interaction between the viable- and the lethal-yellow genes. The viable-yellow gene was mapped to a 58 kb region on chromosome 2 that contained seven predicted genes. A frame shift mutation, due to a single base deletion in <em>Glyma.02g233700,</em> resulted in an early stop codon<em>. Glyma.02g233700</em> encodes a translocon in the inner membrane of chloroplast (GmTic110) that plays a critical role in plastid biogenesis. The lethal-yellow gene was mapped to an 83 kb region on chromosome 3 that contained 13 predicted genes. Based on the annotated functions, we sequenced three potential candidate genes. A single base insertion in the second exon of <em>Glyma.03G230300</em> resulted in a truncated protein. <em>Glyma.03G230300</em> encodes for GmPsbP, an extrinsic protein of Photosystem II that is critical for oxygen evolution during photosynthesis. <em>GmTic110</em> and <em>GmPsbP</em> displayed highly reduced expression in the viable- and lethal-yellow mutants, respectively. The yellow phenotypes in the viable- and lethal-yellow mutants were due to the loss of function of GmTic110 or GmPsbP resulting in photooxidative stress.</p>
dc.description.comments <p>This article is published as Sandhu, Devinder, Taylor Atkinson, Andrea Noll, Callie Johnson, Katherine Espinosa, Jessica Boelter, Stephanie Abel et al. "Soybean proteins GmTic110 and GmPsbP are crucial for chloroplast development and function." <em>Plant Science</em> 252 (2016): 76-87. doi: <a href="https://doi.org/10.1016/j.plantsci.2016.07.006" target="_blank" title="Persistent link using digital object identifier">10.1016/j.plantsci.2016.07.006</a>.</p>
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dc.identifier archive/lib.dr.iastate.edu/agron_pubs/491/
dc.identifier.articleid 1540
dc.identifier.contextkey 12302014
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath agron_pubs/491
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/4853
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/agron_pubs/491/2016_Goggi_SoybeanProteins.pdf|||Sat Jan 15 00:30:00 UTC 2022
dc.source.uri 10.1016/j.plantsci.2016.07.006
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.keywords Soybean
dc.subject.keywords Chlorophyll-deficient
dc.subject.keywords Photosynthesis
dc.subject.keywords Tic110
dc.subject.keywords PsbP
dc.title Soybean proteins GmTic110 and GmPsbP are crucial for chloroplast development and function
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication 4bb5eaad-e792-462d-8f9d-b83f80fa2e64
relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a
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