Structural and functional effects of Bordetella avium infection in the turkey respiratory tract

dc.contributor.author Van Alstine, William
dc.contributor.department Veterinary Pathology
dc.date 2018-08-23T06:23:25.000
dc.date.accessioned 2020-06-30T07:14:40Z
dc.date.available 2020-06-30T07:14:40Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 1987
dc.date.issued 1987
dc.description.abstract <p>Following aerosol exposure to virulent Escherichia coli, lung and tracheal samples were collected from Bordetella avium-infected (BA+) and B. avium-free (BA-) turkeys for bacterial quantitation and histopathologic examination. Pulmonary clearance of E. coli was not significantly different between BA+ and BA- groups, however numbers of E. coli isolated from trachea were significantly greater in BA+ turkeys compared with BA- turkeys. Air sacculitis was more common and more severe in BA+ turkeys;Tracheal adherence and colonization by E. coli were determined using an in vivo bacterial adherence assay. Compared with BA- turkeys, BA+ turkeys had significantly greater numbers of E. coli in their tracheas. Mechanisms for adherence and colonization by E. coli were not found;Pulmonary lesions and localization of bacteria in the respiratory tract were determined in turkeys experimentally infected with B. avium. Bordetella avium was isolated from trachea and primary bronchi of all BA+ turkeys, but was never isolated from lung parenchyma. Acute purulent bronchitis associated with B. avium colonization of the primary bronchi subsided by 3 weeks postinoculation. Bronchus-associated lymphoid tissue was histologically similar in BA+ and BA- turkeys and consisted of discrete lymphoid nodules, which were more numerous and widely distributed along primary bronchi of BA+ turkeys;Metabolic activity of tracheal epithelium was evaluated using a tetrazolium chloride reduction assay. Morphology of epithelium was determined by light microscopy in turkey tracheal organ cultures (TOCs) exposed to live B. avium, formalin killed B. avium, B. avium sonicate, heat-inactivated sonicate, culture supernatant, heat-inactivated culture supernatant, phosphate buffered saline, or brain heart infusion broth. Although the bacterial sonicate containing heat-labile toxin was lethal for turkeys, neither the sonicate nor any other B. avium fraction significantly affected the metabolism or morphology of turkey TOCs.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/11655/
dc.identifier.articleid 12654
dc.identifier.contextkey 6458351
dc.identifier.doi https://doi.org/10.31274/rtd-180813-10601
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/11655
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/64936
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/11655/r_8716833.pdf|||Fri Jan 14 18:55:08 UTC 2022
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Veterinary Medicine
dc.subject.keywords Veterinary pathology
dc.title Structural and functional effects of Bordetella avium infection in the turkey respiratory tract
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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