Electrical Differentiation of Mesenchymal Stem Cells into Schwann‐Cell‐Like Phenotypes Using Inkjet‐Printed Graphene Circuits

dc.contributor.author Claussen, Jonathan
dc.contributor.author Sakaguchi, Donald
dc.contributor.author Ding, Shaowei
dc.contributor.author Lentner, Matthew
dc.contributor.author Hondred, John
dc.contributor.author Cargill, Allison
dc.contributor.author Sakaguchi, Donald
dc.contributor.author Mallapragada, Surya
dc.contributor.author Claussen, Jonathan
dc.contributor.department Ames National Laboratory
dc.contributor.department Mechanical Engineering
dc.contributor.department Neuroscience
dc.contributor.department Genetics, Development and Cell Biology
dc.contributor.department Chemical and Biological Engineering
dc.date 2019-10-22T18:55:35.000
dc.date.accessioned 2020-06-30T04:02:06Z
dc.date.available 2020-06-30T04:02:06Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 2017
dc.date.issued 2017-04-05
dc.description.abstract <p>Graphene-based materials (GBMs) have displayed tremendous promise for use as neuro-interfacial substrates as they enable favorable adhesion, growth, proliferation, spreading and migration of immobilized cells. Herein we report the first case of the differentiation of Mesenchymal Stem Cells (MSCs) into Schwann Cell (SC) like phenotypes through the application of electrical stimuli from a graphene-based electrode. Electrical differentiation of MSCs into SC like phenotypes is carried out on a flexible, inkjet-printed graphene interdigitated electrode (IDE) circuit that is made highly conductive (sheet resistance < 1 kΩ/☐) via a post-print pulse-laser annealing process. MSCs immobilized on the graphene printed IDEs and electrically stimulated/treated (etMSCs) displayed significant enhanced cellular differentiation and paracrine activity above conventional chemical treatment strategies [~85% of the etMSCs differentiated into SCs like phenotypes with ~80 ng/mL of nerve growth factor (NGF) secretion vs. 75% and ~55 ng/mL for chemically treated MSCs (ctMSCs)]. These results help pave the way for in vivo peripheral nerve regeneration where the flexible This article is protected by copyright. All rights reserved. 3 graphene electrodes could conform to the injury site and provide intimate electrical simulation for nerve cell regrowth.</p>
dc.description.comments <p>This is the peer reviewed version of the following article: Das, Suprem R., Metin Uz, Shaowei Ding, Matthew T. Lentner, John A. Hondred, Allison A. Cargill, Donald S. Sakaguchi, Surya Mallapragada, and Jonathan C. Claussen. "Electrical differentiation of mesenchymal stem cells into Schwann‐cell‐like phenotypes using inkjet‐printed graphene circuits." <em>Advanced healthcare materials</em> 6, no. 7 (2017): 1601087, which has been published in final form at doi: <a href="https://doi.org/10.1002/adhm.201601087">10.1002/adhm.201601087</a>. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/gdcb_las_pubs/231/
dc.identifier.articleid 1237
dc.identifier.contextkey 15601582
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath gdcb_las_pubs/231
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/37907
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/gdcb_las_pubs/231/2017_Sakaguchi_ElectricalDifferentiationManucript.pdf|||Fri Jan 14 22:47:36 UTC 2022
dc.source.uri 10.1002/adhm.201601087
dc.subject.disciplines Bioelectrical and Neuroengineering
dc.subject.disciplines Cell Biology
dc.subject.disciplines Developmental Biology
dc.subject.keywords Mesenchymal stem cell differentiation
dc.subject.keywords Schwann cells
dc.subject.keywords graphene
dc.subject.keywords printable electronics
dc.subject.keywords nerve cell growth
dc.title Electrical Differentiation of Mesenchymal Stem Cells into Schwann‐Cell‐Like Phenotypes Using Inkjet‐Printed Graphene Circuits
dc.type article
dc.type.genre article
dspace.entity.type Publication
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