Human Respiratory Syncytial Virus Memphis 37 Grown in HEp-2 Cells Causes more Severe Disease in Lambs than Virus Grown in Vero Cells

dc.contributor.author Gallup, Jack
dc.contributor.author Derscheid, Rachel
dc.contributor.author Van Geelen, Albert
dc.contributor.author McGill, Jodi
dc.contributor.author Ackermann, Mark
dc.contributor.author Gallup, Jack
dc.contributor.author Cihlar, tomas
dc.contributor.author Sacco, Randy
dc.contributor.author Ackermann, Mark
dc.contributor.department Veterinary Pathology
dc.date 2018-02-13T19:20:40.000
dc.date.accessioned 2020-07-07T05:15:58Z
dc.date.available 2020-07-07T05:15:58Z
dc.date.copyright Tue Jan 01 00:00:00 UTC 2013
dc.date.embargo 2014-01-03
dc.date.issued 2013-11-22
dc.description.abstract <p>Respiratory syncytial virus (RSV) is the most common cause of bronchiolitis in infants and young children. A small percentage of these individuals develop severe and even fatal disease. To better understand the pathogenesis of severe disease and develop therapies unique to the less-developed infant immune system, a model of infant disease is needed. The neonatal lamb pulmonary development and physiology is similar to that of infants, and sheep are susceptible to ovine, bovine, or human strains of RSV. RSV grown in Vero (African green monkey) cells has a truncated attachment G glycoprotein as compared to that grown in HEp-2 cells. We hypothesized that the virus grown in HEp-2 cells would cause more severe clinical symptoms and cause more severe pathology. To confirm the hypothesis, lambs were inoculated simultaneously by two different delivery methods (intranasal and nebulized inoculation) with either Vero-grown or HEp-2-grown RSV Memphis 37 (M37) strain of virus to compare viral infection and disease symptoms. Lambs infected with HEp-2 cell-derived virus by either intranasal or nebulization inoculation had significantly higher levels of viral RNA in lungs as well as greater clinical disease including both gross and histopathologic lesions compared to lambs similarly inoculated with Vero-grown virus. Thus, our results provide convincing <em>in vivo</em> evidence for differences in viral infectivity that corroborate previous <em>in vitro</em> mechanistic studies demonstrating differences in the G glycoprotein expression by RSV grown in Vero cells.</p>
dc.description.comments <p>This article is from <em>Viruses</em> 5 (2013): 2881–2897, doi:<a href="http://dx.doi.org/10.3390/v5112881" target="_blank">10.3390/v5112881</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vpath_pubs/52/
dc.identifier.articleid 1054
dc.identifier.contextkey 4952341
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vpath_pubs/52
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92478
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vpath_pubs/52/2013_DerscheidRJ_HumanRespiratorySyncytial.pdf|||Sat Jan 15 00:46:07 UTC 2022
dc.source.uri 10.3390/v5112881
dc.subject.disciplines Veterinary Infectious Diseases
dc.subject.disciplines Veterinary Pathology and Pathobiology
dc.subject.keywords G protein
dc.subject.keywords HEp-2
dc.subject.keywords Infant
dc.subject.keywords lamb
dc.subject.keywords lung
dc.subject.keywords respiratory syncytial virus (RSV)
dc.subject.keywords Vero
dc.title Human Respiratory Syncytial Virus Memphis 37 Grown in HEp-2 Cells Causes more Severe Disease in Lambs than Virus Grown in Vero Cells
dc.type article
dc.type.genre article
dspace.entity.type Publication
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relation.isAuthorOfPublication 86c1ed73-b60d-48ce-8f35-449bc320a693
relation.isOrgUnitOfPublication cf38d7e3-b5f8-4859-83e3-ae8fab6a4c5f
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