Investigation of mechanisms of action of monoterpenoid insecticides on insect gamma-aminobutyric acid receptors and nicotinic acetylcholine receptors

dc.contributor.advisor Joel R. Coats
dc.contributor.author Tong, Fan
dc.contributor.department Entomology
dc.date 2018-08-11T13:12:43.000
dc.date.accessioned 2020-06-30T02:37:18Z
dc.date.available 2020-06-30T02:37:18Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 2010
dc.date.embargo 2013-06-05
dc.date.issued 2010-01-01
dc.description.abstract <p>Monoterpenoids and their derivatives from plant essential oils show potential to be a good alternative to conventional insecticides due to their relatively high toxicity to insect pests, low toxicity to non-target organisms, and biodegradability in the environment. This dissertation has investigated the possible mechanisms of action of monoterpenoid insecticides in the insect nervous system, including an inhibitory neurotransmitter receptor, the GABA receptor, and an excitatory neurotransmitter, the nicotinic acetylcholine receptor (nAChR). By using radioligand binding assays, 36Cl- uptake assays, and quantitative structure-activity relationship assays, monoterpenoid insecticides were suggested to bind to these two receptors, and could interfere with the function of them in the nervous system, which might be the reason why these naturally occurring compounds have toxicity to insects. In the Chapter 2, carvacrol, pulegone, and thymol showed binding activities on insect GABA receptor, and inhibitory effects on the insect nervous system. In the Chapter 3, some structural and chemical properties of monoterpenoids, including Mulliken charges, log P values, and total energy levels, were indicated to be related to the binding between monoterpenoids and the insect GABA receptor. In the Chapter 4, a potent monoterpenoid insecticide, carvacrol, showed binding activity on insect nAChR at a new binding site. This dissertation will help us to understand the mechanism of action of monoterpenoid insecticides further, and is meaningful to discover and develop novel green insecticides.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/11665/
dc.identifier.articleid 2726
dc.identifier.contextkey 2807924
dc.identifier.doi https://doi.org/10.31274/etd-180810-48
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/11665
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/25871
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/11665/Tong_iastate_0097E_11493.pdf|||Fri Jan 14 18:55:17 UTC 2022
dc.subject.disciplines Entomology
dc.subject.keywords GABA receptor
dc.subject.keywords monoterpenoid
dc.subject.keywords nAChR
dc.title Investigation of mechanisms of action of monoterpenoid insecticides on insect gamma-aminobutyric acid receptors and nicotinic acetylcholine receptors
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication f47c8cad-50be-4fb0-8870-902ff536748c
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
File
Original bundle
Now showing 1 - 1 of 1
Name:
Tong_iastate_0097E_11493.pdf
Size:
1.11 MB
Format:
Adobe Portable Document Format
Description: