Porcine IgA allotypes are not equally transcribed or expressed in heterozygous swine

dc.contributor.author Navarro, P.
dc.contributor.author Christensen, R. K.
dc.contributor.author Rothschild, Max
dc.contributor.author Weber, P.
dc.contributor.author Rothschild, Max
dc.contributor.author Ekhardt, G.
dc.contributor.author Butler, J. E.
dc.contributor.department Animal Science
dc.date 2018-02-17T11:58:30.000
dc.date.accessioned 2020-06-29T23:39:33Z
dc.date.available 2020-06-29T23:39:33Z
dc.date.issued 2000-01-01
dc.description.abstract <p>The prediction of 1:1 expression of constant region allotypes in heterozygous animals assumes that productive VDJ rearrangements occur at random between chromosomes, switch recombination is random, there is no allele-related defect in switching and there is no selection for a B-cell receptor bearing a certain constant region allotype. In data reported here, this prediction was often not fulfilled for the transcripts encoding the IgA<sup>a</sup> and IgA<sup>b</sup> alleles of porcine IgA including those from late term fetal piglets that are not in contact with environmental antigens or maternal regulatory factors. In the spleen, thymus, mesenteric lymph node (MLN), ileal Peyer patches, parotid gland and PBLs of 5-week-old conventionally-reared Duroc pigs, ratios of IgA<sup>a</sup> to IgA<sup>b</sup> transcripts as high as 4:1 were observed. Since White Cross animals had significantly higher levels of IgA<sup>b</sup> than IgA<sup>a</sup> (some >3-fold), a allele-linked switch defect cannot explain the deviation from the expected 1:1 ratio. When the IgA<sup>a</sup>:IgA<sup>b</sup> ratios in older Durocs and those reared at a different site were studied, no evidence for breed dependence of differential transcription was found. Total serum IgA levels paralleled total transcript levels in PBLs while particularly in White Cross animals, the IgA<sup>a</sup>:IgA<sup>b</sup> ratio in serum was higher in many animals than the IgA<sup>a</sup>:IgA<sup>b</sup> transcript ratio in their PBLs. We conclude that deviations from the expected 1:1 ratio of allotype transcripts and secreted IgA in young pigs is normal and deviations from this ratio also occur during fetal life in the absence of environmental antigens and maternal regulatory factors. We speculate that postnatal deviations result from: (a) exposure to environmental antigens that selectively expand B-cells expressing V<sub>H</sub> gene alleles linked to either IgA<sup>a</sup> or IgA<sup>b</sup> or (b) some form of colostrum-dependent regulation. Pre-natal regulation may depend on the selection of B-cells bearing certain V<sub>H</sub> or C<sub>H</sub> encoded BCRs by stromal ligands such as fetal B-cell superantigens.</p>
dc.description.comments <p>This is an article from <em>Molecular Immunology</em> 37 (2000): 653, doi:<a href="http://dx.doi.org/10.1016/S0161-5890(00)00086-9" target="_blank">10.1016/S0161-5890(00)00086-9</a>. Posted with permission.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/ans_pubs/277/
dc.identifier.articleid 1266
dc.identifier.contextkey 8089587
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath ans_pubs/277
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/9688
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/ans_pubs/277/2000_Rothschild_PorcineIgA.pdf|||Fri Jan 14 23:07:47 UTC 2022
dc.source.uri 10.1016/S0161-5890(00)00086-9
dc.subject.disciplines Agriculture
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Microbiology
dc.subject.keywords Porcine IgA allotypes
dc.subject.keywords Transcribed
dc.subject.keywords Expressed
dc.subject.keywords Heterozygous swine
dc.title Porcine IgA allotypes are not equally transcribed or expressed in heterozygous swine
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication a5915699-0c8a-411e-a601-1564d7090ab9
relation.isOrgUnitOfPublication 85ecce08-311a-441b-9c4d-ee2a3569506f
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