Induction of central venous catheter-associated sepsis (CAS): A novel sheep model simulating the complex pathophysiology observed in humans. A paper to be submitted to Shock. Beal, Matthew
dc.contributor.department Veterinary Microbiology and Preventive Medicine
dc.contributor.majorProfessor Dr. Paul Plummer 2020-03-02T19:34:20.000 2020-06-30T01:35:07Z 2020-06-30T01:35:07Z Mon Jan 01 00:00:00 UTC 2018 2018-01-01
dc.description.abstract <p>Catheter-associated blood stream infections (CABSIs) are serious, yet common, outcomes in both human and animals with indwelling catheters. The increasing rate of these infections is partially due to hindered knowledge on how to stop the spred of these pathogens, this due, in part, to inadequate animal models. Current experimental models fail to mimic various aspects of sepsis pathogenesis, diverse clinical symptomology, and in most cases lack the ability to test novel therapies for use in human medicine. In response to the urgent need for a more clinically relevant animal model of CAS, this large animal model was conceptualized and validated through this prospective study. Eight clinically healthy domestic cross-bred mature female sheep were obtained and double-lumen peripherally inserted central catheters (PICCs) were nonsurgically placed in the left jugular veins. A novel inoculation method was developed using a luminal volume of <em>Klebsiella pneumoniae subsp. pneumoniae</em> <em>43816Rif<sup>R</sup></em>isolate and blood mixture (1:3). The mixture was injected into the lumen and was allowed to clot where is remainded for the duration of the study, enabling the development of a chronic catheter infection with the slow prolonged release of bacteria. This continual shedding of bacteria more closely mimicks the natural pathogenesis of catheter-associated sepsis (CAS) in clinical settings. A diverse yet dose-dependent host immune response was observed including; tachypneas, tachycardias, pyrexias, leukopeneas, neutrophilias, thrombocytopenias, hyperlacemias, and in some sheep biochemical signs of organ injury/damage with SOFA scores reaching ≥5. Please see paper for full abstract.</p>
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dc.identifier.articleid 1120
dc.identifier.contextkey 13393401
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath creativecomponents/449
dc.source.bitstream archive/|||Sat Jan 15 00:19:26 UTC 2022
dc.subject.disciplines Animal Diseases
dc.subject.disciplines Animal Experimentation and Research
dc.subject.disciplines Animals
dc.subject.disciplines Bacteria
dc.subject.disciplines Bacteriology
dc.subject.disciplines Comparative and Laboratory Animal Medicine
dc.subject.disciplines Disease Modeling
dc.subject.disciplines Emergency Medicine
dc.subject.disciplines Immune System Diseases
dc.subject.disciplines Immunity
dc.subject.disciplines Immunology of Infectious Disease
dc.subject.disciplines Immunopathology
dc.subject.disciplines Laboratory and Basic Science Research
dc.subject.disciplines Medical Immunology
dc.subject.disciplines Medical Microbiology
dc.subject.disciplines Molecular Biology
dc.subject.disciplines Other Animal Sciences
dc.subject.disciplines Pathogenic Microbiology
dc.subject.disciplines Veterinary Microbiology and Immunobiology
dc.subject.keywords large animal sepsis models
dc.subject.keywords catheter-associated sepsis
dc.subject.keywords ovine sepsis model
dc.subject.keywords central catheter sepsis
dc.subject.keywords sepsis pathophysiology
dc.title Induction of central venous catheter-associated sepsis (CAS): A novel sheep model simulating the complex pathophysiology observed in humans. A paper to be submitted to Shock.
dc.type article
dc.type.genre creativecomponent
dspace.entity.type Publication
relation.isOrgUnitOfPublication 16f8e472-b1cd-4d8f-b016-09e96dbc4d83 Veterinary Microbiology creativecomponent
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