Pseudorabies (Aujeszky's disease) virus DNA detection in swine nasal swab and oral fluid specimens using a gB-based real-time quantitative PCR

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dc.contributor.author Cheng, Ting-Yu
dc.contributor.author Henao-Diaz, Alexandra
dc.contributor.author Poonsuk, Korakrit
dc.contributor.author Wang, Chong
dc.contributor.author Buckley, Alexandra
dc.contributor.author van Geelen, Albert
dc.contributor.author Lager, Kelly
dc.contributor.author Harmon, Karen
dc.contributor.author Gauger, Phillip
dc.contributor.author Wang, Chong
dc.contributor.author Ambagala, Aruna
dc.contributor.author Zimmerman, Jeffrey
dc.contributor.author Giménez-Lirola, Luis
dc.contributor.department Statistics
dc.contributor.department Veterinary Diagnostic and Production Animal Medicine
dc.date 2021-03-04T00:30:22.000
dc.date.accessioned 2021-04-30T12:24:16Z
dc.date.available 2021-04-30T12:24:16Z
dc.date.issued 2021-04-01
dc.description.abstract <p>In this study, the detection of <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/pseudorabies" title="Learn more about PRV from ScienceDirect's AI-generated Topic Pages">PRV</a> DNA in nasal swab (<em>n</em> = 440) and oral fluid (<em>n</em> = 1,545) samples collected over time from experimentally PRV vaccinated and/or PRV inoculated pigs (<em>n</em> = 40) was comparatively evaluated by real-time PCR. Serum samples (<em>n</em> = 440) were tested by PRV gB/gE blocking ELISAs (Pseudorabies Virus gB Antibody Test Kit and Pseudorabies Virus gpI Antibody Test Kit, IDEXX Laboratories, Inc., Westbrook, ME) to monitor PRV status over time. Following exposure to a gE-deleted modified live vaccine (Ingelvac® Aujeszky MLV, Boehringer Ingelheim, Ridgefield, CT) and/or a wild-type virus (3 CR Ossabaw), PRV gB DNA was detected in oral fluid specimens in a pattern similar to that of nasal swabs. For quantitative analyses, PRV PCR quantification cycle (Cq) results were re-expressed as “efficiency standardized Cqs (ECqs)” as a function of PCR efficiency using plate-specific positive amplification controls. ROC analyses of the PRV gB PCR ECqs results showed a similar performance of the PRV gB PCR for nasal swab and oral fluid specimens (area under the ROC curve = 85 % vs 83 %) and, based on an ECq cutoff of 0.01 a diagnostic specificity of 100 % and diagnostic sensitivities for oral fluid and nasal swab specimens of 53 % (95 % CI: 43 %, 62 %) and 70 % (95 % CI: 55 %, 83 %), respectively. Thus, the results described herein demonstrated the detection of PRV gB DNA in swine oral fluid and supported the use of this specimen in PRV diagnosis and surveillance.</p>
dc.description.comments <p>This is article is published as Cheng, Ting-Yu, Alexandra Henao-Diaz, Korakrit Poonsuk, Alexandra Buckley, Albert van Geelen, Kelly Lager, Karen Harmon, Phillip Gauger, Chong Wang, Aruna Ambagala, Jeffrey Zimmerman, and Luis Giménez-Lirola. "Pseudorabies (Aujeszky's disease) virus DNA detection in swine nasal swab and oral fluid specimens using a gB-based real-time quantitative PCR." <em>Preventive Veterinary Medicine</em> 189 (2021): 105308. DOI: <a href="https://doi.org/10.1016/j.prevetmed.2021.105308" target="_blank">10.1016/j.prevetmed.2021.105308</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/vdpam_pubs/202/
dc.identifier.articleid 1206
dc.identifier.contextkey 21929610
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath vdpam_pubs/202
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/105179
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/vdpam_pubs/202/2021_WangChong_PseudorabiesAujeszkys.pdf|||Fri Jan 14 22:21:24 UTC 2022
dc.source.uri 10.1016/j.prevetmed.2021.105308
dc.subject.disciplines Large or Food Animal and Equine Medicine
dc.subject.disciplines Veterinary Preventive Medicine, Epidemiology, and Public Health
dc.subject.keywords qPCR
dc.subject.keywords Oral fluid
dc.subject.keywords Pseudorabies virus
dc.subject.keywords Data standardization
dc.title Pseudorabies (Aujeszky's disease) virus DNA detection in swine nasal swab and oral fluid specimens using a gB-based real-time quantitative PCR
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication b715071c-c3bd-419c-b021-0ac4702f346a
relation.isOrgUnitOfPublication 264904d9-9e66-4169-8e11-034e537ddbca
relation.isOrgUnitOfPublication 5ab07352-4171-4f53-bbd7-ac5d616f7aa8
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