Isolation of Probiotic Bacterial Strains with Potential Anti-Clostridioides difficile Properties from Horse Feces

Date
2022-05
Authors
Jaycie, Pergler
Major Professor
Mooyottu, Shankumar
Greenlee, Heather
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Mooyottu, Shankumar
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Abstract
Clostridioides difficile is a major equine enteric pathogen that causes severe colitis and death in infected animals. Currently, antibiotics are the treatment of choice for equine C. difficile infection (CDI) despite the concerns about antibiotic-induced intestinal complications and the emerging antibiotic resistance in C. difficile. The current study aims at isolating candidate probiotic bacterial strains from healthy horses that could grow in similar growth conditions along with C. difficile and withstand C. difficile predisposing antibiotic pressure. For this, fecal samples from fifty horses were screened for anaerobic bacteria in C. difficile Moxalactam Norfloxacin Taurocholate media (CDMNT), and the identity of the isolates was confirmed by MALDI-TOF. Our screening method yielded six different bacterial genera, which included Lactobacillus, Clostridia, and Enterococcus. Among these five species of Lactobacillus have been identified to have probiotic potential as per published literature. Therefore, these bacterial isolates can be tested for their anti-CDI properties to control CDI in horses as an antibiotic alternative.
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