Gametophytic cross-incompatibility in maize: resequencing the Ga1 locus

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dc.contributor.advisor M. Paul Scott
dc.contributor.advisor Thomas Lübberstedt
dc.contributor.author Emery, Marianne
dc.contributor.department Agronomy
dc.date 2018-08-11T15:51:24.000
dc.date.accessioned 2020-06-30T02:58:12Z
dc.date.available 2020-06-30T02:58:12Z
dc.date.copyright Thu Jan 01 00:00:00 UTC 2015
dc.date.embargo 2001-01-01
dc.date.issued 2015-01-01
dc.description.abstract <p>Maize is an important staple crop for many countries. Culture dictates maize use, processing, and incorporation into foods. The crop has a rich history of domestication and improvement. With its relative ease of genetic manipulation, maize is considered a model crop for plant genetic experimentation. Recent biotechnological advances, as well as the completed B73 reference genome sequence, have expedited maize improvement. One such profound advance that has greatly increased profitability of maize is the use of transgenes. Despite the many benefits, transgenic plants are problematic when they contaminate transgene-free maize. Maintaining the purity of transgene-free maize is crucial, but often difficult when in close proximity to transgenic fields. Past literature suggests the use of the Ga1 gametophytic cross-incompatibility system to control pollen flow and minimize contamination of transgene-free maize. Yet, information about how the gametophytic cross-incompatibility system functions at the molecular level is still lacking. Our research sought to assemble BACs containing the Ga1-m locus to better understand sequence variation with the B73 reference genome that may be causative of the male function in the Ga1 gametophytic cross-incompatibility system.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/14651/
dc.identifier.articleid 5658
dc.identifier.contextkey 8052010
dc.identifier.doi https://doi.org/10.31274/etd-180810-4204
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/14651
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/28836
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/14651/Emery_iastate_0097M_15016.pdf|||Fri Jan 14 20:24:09 UTC 2022
dc.subject.disciplines Agricultural Science
dc.subject.disciplines Agriculture
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Genetics
dc.subject.keywords Plant Breeding
dc.subject.keywords assembly
dc.subject.keywords BAC
dc.subject.keywords cross-incompatibility
dc.subject.keywords gametophytic
dc.subject.keywords maize
dc.subject.keywords resequencing
dc.title Gametophytic cross-incompatibility in maize: resequencing the Ga1 locus
dc.type article
dc.type.genre thesis
dspace.entity.type Publication
relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a
thesis.degree.level thesis
thesis.degree.name Master of Science
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