Function and application of TAL effectors

dc.contributor.advisor Bing Yang
dc.contributor.author Li, Ting
dc.contributor.department Genetics, Development and Cell Biology
dc.date 2018-08-11T10:29:57.000
dc.date.accessioned 2020-06-30T02:52:46Z
dc.date.available 2020-06-30T02:52:46Z
dc.date.copyright Wed Jan 01 00:00:00 UTC 2014
dc.date.embargo 2001-01-01
dc.date.issued 2014-01-01
dc.description.abstract <p>The transcription activator-like effectors (TALE) are the largest family of the type III effector proteins from Xanthomonas spp. Each TALE contains an N-terminal signal for its secretion and translocation into host cells, a central modular DNA-binding repeat domain, and three nuclear localization signals (NLS) and an acidic activation domain (AD) at the C-terminus. Once internalized, TALEs facilitate the bacterial parasitism by transcriptionally activating the host target genes. TALE repeats recognize and bind to the target DNA sequence in a simple and unique "one to one" fashion, i.e., one repeat corresponds to one nucleotide and one type of repeat preferentially binds one of four necleotides, a code that makes TALE an ideal material to achieve the recognition of any preselected DNA sequence.</p> <p>In my thesis I describe the development and functional characterization of TALENs, fusion proteins of native or artificial TALEs and the cleavage domain of the restriction enzyme FokI, in vitro and in yeast, and demonstrate the feasibility of customizing the novel TALEN to target and inactivate the yeast endogenous genes. I also investigated the efficiency of the gene editing through the repair of TALEN-caused double-stranded DNA breaks by non-homologous end-joining (NHEJ) and homologous recombination (HR). Furthermore, I utilized the designer TALENs to edit the TAL effector binding elements (EBEs) in the promoters of the disease susceptibility (S) genes targeted by the rice blight pathogen Xanthomonas oryzae pv. oryzae (Xoo). The gene editing disrupted the induction of the S genes by the naturally occurring TALEs (PthXo1 and AvrXa7) and thus generated the disease resistant rice lines. Finally, using Xoo-delivered designer TALEs to induce the expression of rice SWEET genes that encode sugar transporters in rice, I identified two disease susceptibility genes potentially targeted by the as yet unidentified TALEs in Xanthomonas oryzae pv. oryzae.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/etd/13875/
dc.identifier.articleid 4882
dc.identifier.contextkey 5777589
dc.identifier.doi https://doi.org/10.31274/etd-180810-850
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath etd/13875
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/28062
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/etd/13875/Li_iastate_0097E_14045.pdf|||Fri Jan 14 20:02:55 UTC 2022
dc.subject.disciplines Genetics
dc.subject.keywords genome editing
dc.subject.keywords genome modification
dc.subject.keywords TAL effector
dc.subject.keywords TAL effector nuclease
dc.title Function and application of TAL effectors
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 9e603b30-6443-4b8e-aff5-57de4a7e4cb2
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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