Triple pulse amperometry: a method of detection of carbohydrates and alcohols for liquid chromatography

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dc.contributor.author Hughes, Scott
dc.contributor.department Chemistry
dc.date 2018-08-15T21:50:29.000
dc.date.accessioned 2020-07-02T06:00:56Z
dc.date.available 2020-07-02T06:00:56Z
dc.date.copyright Fri Jan 01 00:00:00 UTC 1982
dc.date.issued 1982
dc.description.abstract <p>Application of a triple pulse waveform is described for the anodic detection of simple alcohols and carbohydrates at a platinum electrode. The execution of the waveform incorporates the cleaning and reactivation of the platinum electrode, by alternate anodic and cathodic polarization, with measurement of the faradaic signal for the analyte at the reduced electrode surface. The waveform is completed within 0.5-1.5 s and the sampled faradaic signal exhibits no decay with time as would be the case for amperometric detection at a constant applied potential. Calibration curves made by plotting -1/I vs. 1/C are linear. This observation is consistent with a reaction mechanism in which the analyte is adsorbed prior to anodic detection;The technique was applied at a miniature platinum wire-tip electrode in a flow-through electrochemical detector for the determination of alcohols and carbohydrates separated by HPLC. A detection limit of 0.46 (mu)g (signal-to-noise = 2) was determined for glucose. The analysis of several real samples, including wine, beer and urine, is described for carbohydrates and alcohol.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/7506/
dc.identifier.articleid 8505
dc.identifier.contextkey 6314481
dc.identifier.doi https://doi.org/10.31274/rtd-180813-6253
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/7506
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/80391
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/7506/r_8224224.pdf|||Sat Jan 15 01:49:39 UTC 2022
dc.subject.disciplines Analytical Chemistry
dc.subject.keywords Chemistry
dc.subject.keywords Analytic
dc.subject.keywords Chemistry
dc.title Triple pulse amperometry: a method of detection of carbohydrates and alcohols for liquid chromatography
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
relation.isOrgUnitOfPublication 42864f6e-7a3d-4be3-8b5a-0ae3c3830a11
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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