Method for aerosolization and collection of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): engineering considerations

Date
2021-01-01
Authors
Li, Peiyang
Koziel, Jacek
Zimmerman, Jeffrey
Hoff, Steven
Zhang, Jianqiang
Cheng, Ting-Yu
Yim-Im, Wannarat
Lee, Myeongseong
Chen, Baitong
Jenks, William
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Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) infections cause significant economic losses to swine producers every year. Aerosols containing infectious PRRSV are an important route of transmission, and proper treatment of air could mitigate the airborne spread of the virus within and between barns. Previous bioaerosol studies focused on the microbiology of PRRSV aerosols; thus, the current study addressed the engineering aspects of virus aerosolization and collection. Specific objectives were to (1) build and test a virus aerosolization system, (2) achieve a uniform and repeatable aerosol generation and collection throughout all replicates, (3) identify and minimize sources of variation, (4) verify that the collection system (impingers) performed similarly. The system for virus aerosolization was built and tested (Obj. 1). The uniform airflow distribution was confirmed using a physical tracer (

Description

This conference presentation is published as Li, Peiyang, Jacek A. Koziel, Jeffrey J. Zimmerman, Steven J. Hoff, Jianqiang Zhang, Ting-Yu Cheng, Wannrat Yim-Im, Myeongseong Lee, Baitong Chen, and William S. Jenks. "Method for aerosolization and collection of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): engineering considerations." ASABE Paper No. 2100152. ASABE Annual International Meeting, July 12-16, 2021. DOI: 10.13031/aim.202100152. Posted with permission.

Keywords
airborne pathogens, animal production, infectious animal disease, livestock health, mass balance, swine diseases, viral aerosol, virus isolation
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