Tissue-specific patterns of a maize Myb transcription factor are epigenetically regulated

dc.contributor.author Cocciolone, Suzy
dc.contributor.author Peterson, Thomas
dc.contributor.author Chopra, Surinder
dc.contributor.author Flint-Garcia, Sherry
dc.contributor.author McMullen, Michael
dc.contributor.author Peterson, Thomas
dc.contributor.department Zoology and Genetics
dc.contributor.department Agronomy
dc.date 2018-02-18T10:48:09.000
dc.date.accessioned 2020-07-07T05:16:57Z
dc.date.available 2020-07-07T05:16:57Z
dc.date.issued 2001-09-01
dc.description.abstract <p>The maize <em>p1</em> gene encodes a <em>Myb</em>-homologous regulator of red pigment biosynthesis. To investigate the tissue-specific regulation of the <em>p1</em> gene, maize plants were transformed with constructs combining promoter and cDNA sequences of two alleles which differ in pigmentation patterns: <em>P1-wr</em> (white pericarp/red cob) and <em>P1-rr</em> (red pericarp/red cob). Surprisingly, all promoter/cDNA combinations produced transgenic plants with red pericarp and red cob (RR pattern), indicating that the <em>P1-wr</em> promoter and encoded protein can function in pericarp. Some of the RR patterned transgenic plants produced progeny plants with white pericarp and red cob (WR pattern), and this switch in tissue-specificity correlated with increased transgene methylation. A similar inverse correlation between pericarp pigmentation and DNA methylation was observed for certain natural <em>p1</em> alleles, which have a gene structure characteristic of standard <em>P1-wr</em> alleles, but which confer red pericarp pigmentation and are consistently less methylated than standard <em>P1-wr</em> alleles. Although we cannot rule out the possible existence of tissue-specific regulatory elements within the <em>p1</em> non-coding sequences or flanking regions, the data from transgenic and natural alleles suggest that the tissue-specific pigmentation pattern characteristic of the <em>P1-wr</em>phenotype is epigenetically controlled.</p>
dc.description.comments <p>This article is from <em>The Plant Journal </em>27 (2001): 467, doi: <a href="http://dx.doi.org/10.1046/j.1365-313X.2001.01124.x" target="_blank">10.1046/j.1365-313X.2001.01124.x</a>.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/zool_pubs/41/
dc.identifier.articleid 1039
dc.identifier.contextkey 10168663
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath zool_pubs/41
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/92652
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/zool_pubs/41/2001_Peterson_TissueSpecific.pdf|||Sat Jan 15 00:09:58 UTC 2022
dc.source.uri 10.1046/j.1365-313X.2001.01124.x
dc.subject.disciplines Agronomy and Crop Sciences
dc.subject.disciplines Cell and Developmental Biology
dc.subject.disciplines Genetics and Genomics
dc.subject.disciplines Plant Breeding and Genetics
dc.subject.keywords epigenetic
dc.subject.keywords maize
dc.subject.keywords transgenic plants
dc.subject.keywords tissue-specific
dc.subject.keywords pericarp
dc.subject.keywords flavonoids
dc.title Tissue-specific patterns of a maize Myb transcription factor are epigenetically regulated
dc.type article
dc.type.genre article
dspace.entity.type Publication
relation.isAuthorOfPublication ce966761-2eda-46df-bdb8-30787bedf687
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relation.isOrgUnitOfPublication fdd5c06c-bdbe-469c-a38e-51e664fece7a
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