Characterization of a rat clonal [beta]-cell line, RINm5F, in a passage- and morphology-dependent manner

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dc.contributor.advisor Walter H. Hsu
dc.contributor.author Lee, Bum-sup
dc.contributor.department Veterinary Physiology and Pharmacology
dc.date 2018-08-23T07:15:58.000
dc.date.accessioned 2020-06-30T07:09:53Z
dc.date.available 2020-06-30T07:09:53Z
dc.date.copyright Sun Jan 01 00:00:00 UTC 1995
dc.date.issued 1995
dc.description.abstract <p>A novel insulin-like immunoreactivity, adrenergic receptors (ARs) and voltage-dependent Ca[superscript]2+ channels (VDCCs) were characterized in a clonal [beta]-cell line, RINm5F, in a passage- and morphology-dependent manner. The cells exhibited two morphological features; the differentiated cells (DCs) with long neuron-like processes and the undifferentiated cells (UCs) without the processes;As morphology of the cells changed from DCs to UCs, amount of secreted insulin gradually decreased; however, insulin reacted to antiserum vs. bovine insulin (ABI) gradually increased from barely detectable amounts. Immunoblotting and immunocytochemistry showed that antiserum vs. rat insulin reacted with insulin from both cell types. ABI did not react with insulin from DCs but from UCs;cAMP production and insulin release by epinephrine were decreased in DCs but increased in UCs. In DCs, epinephrine decreased KCl-induced insulin release but RX 821002 abolished the epinephrine-induced inhibition. Prazosin and propranolol failed to alter epinephrine's effect. In UCs, however, epinephrine enhanced KCl's action. RX 821002 further potentiated the effect of epinephrine on KCl's action. Propranolol abolished the effect of epinephrine on KCl's action but prazosin did not;Cholera toxin equally increased cAMP production in DCs and UCs. Effects of forskolin and IBMX on cAMP production were greater in UCs than in DCs. Activity of protein kinase A was not significantly different between DCs and UCs;Effects of Bay K 8644 and nimodipine on Ca[superscript]2+ currents, (Ca[superscript]2+) [subscript] i and insulin release were greater in DCs than in UCs. Nimodipine (1 [mu]M) failed to inhibit control Ca[superscript]2+ currents at the holding potentials of -80 mV but inhibited them by ~70% at O mV. The current-voltage relationship for activation and inactivation of Ca[superscript]2+ currents shifted to more negative potentials in DCs than in UCs;In summary, these findings suggested that a novel insulin-like immunoreactivity is present in UCs, which is not due to rat insulin I nor II. DCs have greater activity of [alpha][subscript]2-, but lesser activity of [beta]-ARs than UCs. Increased activity of [beta]-ARs in UCs is due partly to increased activity of adenylyl cyclase. DCs have greater activity of L-type VDCCs than UCs. The voltage-dependence of L-type VDCCs was different between DCs and UCs.</p>
dc.format.mimetype application/pdf
dc.identifier archive/lib.dr.iastate.edu/rtd/11067/
dc.identifier.articleid 12066
dc.identifier.contextkey 6430620
dc.identifier.doi https://doi.org/10.31274/rtd-180813-12165
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath rtd/11067
dc.identifier.uri https://dr.lib.iastate.edu/handle/20.500.12876/64283
dc.language.iso en
dc.source.bitstream archive/lib.dr.iastate.edu/rtd/11067/r_9610968.pdf|||Fri Jan 14 18:41:30 UTC 2022
dc.subject.disciplines Animal Sciences
dc.subject.disciplines Cell Biology
dc.subject.disciplines Molecular Biology
dc.subject.disciplines Physiology
dc.subject.disciplines Veterinary Physiology
dc.subject.keywords Veterinary physiology and pharmacology
dc.subject.keywords Physiology (Pharmacology)
dc.subject.keywords Pharmacology
dc.title Characterization of a rat clonal [beta]-cell line, RINm5F, in a passage- and morphology-dependent manner
dc.type article
dc.type.genre dissertation
dspace.entity.type Publication
thesis.degree.level dissertation
thesis.degree.name Doctor of Philosophy
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