Design and Optimization of RSV F for Vaccination Wilson, Elizabeth
dc.contributor.department Chemical and Biological Engineering 2018-02-18T19:50:28.000 2020-06-30T04:34:42Z 2020-06-30T04:34:42Z 2017-04-01
dc.description.abstract <p>Respiratory syncytial virus (RSV) is a common infection that affects most infants by the age of two, as well as a large percentage of the elderly population. Currently, the majority of prevention lies in the hands of the caretakers: wash hands frequently, avoid contact if sick or have babies wear masks, keep young children away from babies, avoid crowds during outbreaks. As these methods are not always feasible, this project aims to develop a vaccine for RSV by focusing on the fusion (F) glycoprotein. This protein is necessary for the virus to infect, as it assists the virus in fusing to the plasma membrane of the target cell, assisting in the injection of viral RNA into target cells and in spreading the virus to other cells. This work aims to manufacture a recombinant F protein, express high quantities of it, and test its immunogenicity in mice. The virus was tested in both eukaryotic and baculovirus cell lines to determine which provided the most optimum immunological response. Additionally, mitochondrial adjuvants (2-D-glucose) were injected in mice to determine their antigenic responses. This step has prepared for future work in testing the response of the F protein in mice.</p>
dc.identifier archive/
dc.identifier.articleid 1191
dc.identifier.contextkey 10582859
dc.identifier.s3bucket isulib-bepress-aws-west
dc.identifier.submissionpath honors_posters/201704/projects/54
dc.relation.ispartofseries Honors Projects and Posters
dc.source.bitstream archive/|||Sat Jan 15 00:53:11 UTC 2022
dc.subject.disciplines Biochemical and Biomolecular Engineering
dc.subject.disciplines Pharmacology
dc.subject.disciplines Respiratory Tract Diseases
dc.subject.disciplines Virus Diseases
dc.title Design and Optimization of RSV F for Vaccination
dc.type event
dc.type.genre event
dspace.entity.type Publication
relation.isOrgUnitOfPublication 86545861-382c-4c15-8c52-eb8e9afe6b75
relation.isSeriesOfPublication 78a1cb49-0dee-4c38-97a8-c1fd0b7a74ea Chemical Engineering
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
446.49 KB
Adobe Portable Document Format